P2x7 receptor antagonists for use in the treatment of inflammatory, immune or cardiovascular diseases

ABSTRACT

The invention provides piperidine compounds of general formula (I) in which A, B, X, Y, Z, R, R 1  and R 2  are as defined in the specification, their use as medicaments, compositions containing them and processes for their for their preparation.

[0001] The present invention relates to piperidine derivatives, a process for their preparation, pharmaceutical compositions containing them, a process for preparing the pharmaceutical compositions, and their use in therapy.

[0002] The P2X₇ receptor (previously known as P2Z receptor), which is a ligand-gated ion channel, is present on a variety of cell types, largely those known to be involved in the inflammatory/immune process, specifically, macrophages, mast cells and lymphocytes (T and B). Activation of the P2X₇ receptor by extracellular nucleotides, in particular adenosine triphosphate, leads to the release of interleukin-1β (IL-1β) and giant cell formation (macrophages/microglial cells), degranulation (mast cells) and L-selectin shedding (lymphocytes). P2X₇ receptors are also located on antigen-presenting cells (APC), keratinocytes, salivary acinar cells (parotid cells) and hepatocytes.

[0003] It would be desirable to make compounds effective as P2X₇ receptor antagonists for use in the treatment of inflammatory, immune or cardiovascular diseases, in the aetiologies of which the P2X₇ receptor may play a role.

[0004] In accordance with the present invention, there is therefore provided a compound of formula (I):

[0005] where

[0006] A is phenyl or a 5- or 6-membered heterocyclic ring containing one or two heteroatoms selected from O, N or S; and optionally substituted by C₁₋₆alkyl, halogen, nitro, amino, alkylamino, CF₃, SO₂Me, NHSO₂Me or cyano;

[0007] B is C═O, NH or SO₂;

[0008] X is C═O, CH(Me), O or (CH₂)p where p is 0 or 1;

[0009] Y is O, CH₂, NH or S;

[0010] Z is C═O or SO₂, provided that when Z is C═O, then Y is O, CH₂ or S;

[0011] R is hydrogen or C₁₋₆alkyl;

[0012] R¹ is hydrogen, halogen;

[0013] R² is phenyl optionally substituted by CO₂H, CO₂alkyl, CONH₂ or R² is OH, NHR³, NHCH(R⁴)(CHR⁵)_(n)R⁶, NH—R⁷—R⁸, SO₂NHalkyl, NHCOalkyl, NHSO₂alkyl, morpholine, NR⁹R¹⁰, piperazine substituted by phenyl, alkoxyphenyl, pyridyl or fluorophenyl;

[0014] n is 0, 1 or 2;

[0015] R³ is hydrogen, a bi- or tricyclic saturated ring system optionally containing a nitrogen atom, piperidinyl, alkylpyrollidine, ethynylcyclohexyl, a 5-membered aromatic ring containing 2 or 3 heteroatoms, C₄₋₆ cycloalkyl optionally substituted by alkyl, cyano or hydroxy, or C₁₋₈alkyl optionally containing an oxygen atom in the alkyl chain and being optionally substituted by one or more substituents selected from ethynyl, cyano, fluoro, di-alkylamino, hydroxy, thioalkyl, CO₂R¹¹ or CONH₂;

[0016] R⁴ is hydrogen or alkyl optionally substituted by hydroxy or alkoxy;

[0017] R⁵ is hydrogen or hydroxy;

[0018] R⁶ is CO₂R¹¹, NHCO₂R¹², CONH₂ or a 5 or 6-membered saturated ring containing an oxygen atom, a 5-membered heterocyclic ring containing one or two heteroatoms selected from O, N or S, or phenyl optionally substituted by one or more groups selected from alkyl, hydroxy, amino, alkoxy, or nitro;

[0019] R⁶ is alkyl;

[0020] R⁷ is a cyclopentane ring;

[0021] R⁸ is phenyl;

[0022] R⁹and R¹⁰ are independently hydrogen, benzyl, alkenyl, cycloalkyl, alkyl optionally substituted by hydroxy, alkoxy, cyano, dialkylamino, phenyl, pyridyl or CO₂R¹¹ or R⁹ and R¹⁰ together form a 5- to 7-membered saturated or partially saturated ring optionally containing a further heteroaton and optionally substituted by one or more groups selected from alkyl (optionally containing an oxygen atom in the chain and optionally substituted by hydroxy), COalkyl, CO₂R¹¹, COR¹³R¹⁴, CHO or piperidine,

[0023] R¹¹ is hydrogen or alkyl;

[0024] R¹² is alkyl; and

[0025] R¹³ and R¹⁴ are independently hydrogen or alkyl, and pharmaceutically acceptable salts and solvates thereof

[0026] In the context of the present specification, unless otherwise indicated, an alkyl substituent or alkyl moiety in a substituent group may be linear or branched and may contain up to 6 carbon atoms, examples of which include methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, t-butyl, n-pentyl and n-hexyl.

[0027] Suitably A is phenyl or a 5- or 6-membered heterocyclic ring containing one or two heteroatoms selected from O, N or S; and optionally substituted by C₁₋₆alkyl, halogen, nitro, amino, alkylamino, CF₃, SO₂Me, NHSO₂Me or cyano. Examples of suitable 5- or 6-membered heterocyclic rings include. Preferably A is optionally substituted phenyl, more preferably A is phenyl substituted by a nitro group.

[0028] Suitably B is C═O, NH or SO₂. Preferably B is C═O.

[0029] Suitably X is C═O, CH(Me), O or (CH₂)p where p is 0 or 1, Y is O, CH₂, NH or S and Z is C═O or SO₂. Examples of groups formed by X, Y and Z include benzoxazinone and dihydroquinoline. Preferably X is CH₂, Y is O and Z is C═O such that X, Y and Z together form part of a benzoxazinone ring which can be optionally substituted by methyl.

[0030] Suitably R is hydrogen or C₁₋₆alkyl, preferably R is hydrogen.

[0031] Suitably R¹ is hydrogen or halogen, preferably R¹ is hydrogen.

[0032] Suitably R² is phenyl optionally substituted by CO₂H, CO₂alkyl, CONH₂ or R² is OH, NHR³, NHCH(R⁴)(CHR⁵)_(n)R⁶, NH—R⁷—R⁸, SO₂NHalkyl, NHCOalkyl, NHSO₂alkyl, morpholine, NR⁹R¹⁰, piperazine substituted by phenyl, alkoxyphenyl, pyridyl or fluorophenyl. Preferably R²is NR⁹R¹⁰ where one of R⁹ or R¹⁰ is hydrogen and the other is alkyl such as CH(CH₃)₂.

[0033] Particularly preferred compounds of the invention include those exemplified herein both in free base form as well as all pharmaceutically acceptable salts and/or solvates thereof

[0034] According to the invention there is further provided a process for the preparation of a compound of formula (I) which comprises reaction of a compound of formula (II):

[0035] where R, R¹, X, Y and Z are as defined in formula (I) or a protected derivative thereof, with a compound of formula (III):

[0036] where B and R² are as defined in formula (I) or a protected derivative thereof, and L is a leaving group, and optionally thereafter in any order:

[0037] converting one or more functional groups into further functional groups

[0038] removing any protecting groups

[0039] forming a pharmaceutically acceptable salt or solvate.

[0040] Examples of suitable leaving groups L include halogen, OMs and OTs. Preferably L is halogen, in particular chloro.

[0041] The reaction of compounds of formula (II) and (III) is preferably carried out in the presence of an organic amine such as a trialkylamine, for example triethylamine. The reaction is preferably carried out in an inert solvent such as NMP, DMF or dioxan preferably at elevated temperature, for example at the reflux temperature of the reaction mixture.

[0042] Compounds of formulae (II) can be prepared as follows:

[0043] (a) by reacting a compound of formula (IV):

[0044]  in which X, Y and Z are as defined in formula (II) or are protected derivatives thereof, with a compound of formula (V):

[0045]  in which R²⁰ is a leaving group or an activated hydroxy group, or

[0046] (b) by reacting a compound of formula (VI):

[0047]  in which P a protecting group, with a compound of formula (VII):

[0048]  in which the groups L are leaving groups.

[0049] Compounds of formulae (IV) and (V) can be reacted under Mitsonobu conditions when R²⁰ in compound (V) is an activated hydroxy group. For the reaction of compounds (VI) and (VII), examples of suitable leaving L groups include halogen, in particular chloro, or imidazole. Alternatively triphosgene can be used. Suitable protecting groups for compounds (V) and (VI) include t-butoxy carbonyl (Boc).

[0050] Compounds of formula (III), (IV), (V) and (VII) are prepared using Iterature procedures or are commercially available.

[0051] Functional groups can be converted into further functional groups using procedures known in the art. For example a carboxylic acid group can be converted into an ester or amide using standard chemistry.

[0052] Protecting groups can be added and removed using known reaction conditions. The use of protecting groups is fully described in ‘Protective Groups in Organic Chemistry’, edited by J W F McOmie, Plenum Press (1973), and ‘Protective Groups in Organic Synthesis’, 2nd edition, T W Greene & P G M Wutz, Wiley-Interscience (1991).

[0053] Deprotection can be carried out using methods generally known in the art.

[0054] All novel intermediates form a further aspect of the invention.

[0055] The compounds of formula (I) above may be converted to a pharmaceutically acceptable salt or solvate thereof, preferably an acid addition salt such as a hydrochloride, hydrobromide, phosphate, acetate, fumarate, maleate, tartrate, citrate, oxalate, methanesulphonate or p-toluenesulphonate, or an alkali metal salt such as a sodium or potassium salt.

[0056] Certain compounds of formula (I) are capable of existing in stereoisomeric forms. It will be understood that the invention encompasses all geometric and optical isomers of the compounds of formula (I) and mixtures thereof including racemates. Tautomers and mixtures thereof also form an aspect of the present invention.

[0057] The compounds of the present invention are advantageous in that they possess pharmacological activity and have utility as modulators of P2X₇ receptor activity. They are therefore indicated as pharmaceuticals for use in the treatment or prevention of rheumatoid arthritis, osteoarthritis, psoriasis, allergic dermatitis, asthma, hyperresponsiveness of the airway, chronic obstructive pulmonary disease (COPD), bronchitis, septic shock, glomerulonephritis, irritable bowel disease, Crohn's disease, ulcerative colitis, atherosclerosis, growth and metastases of malignant cells, myoblastic leukaemia, diabetes, neurodegenerative disease, Alzheimer's disease, meningitis, osteoporosis, burn injury, ischaemic heart disease, stroke, peripheral vascular disease and varicose veins.

[0058] Accordingly, the present invention provides a compound of formula (I), or a pharmaceutically acceptable salt or solvate thereof, as hereinbefore defined for use in therapy.

[0059] In another aspect, the invention provides the use of a compound of formula (I), or a pharmaceutically acceptable salt or solvate thereof, as hereinbefore defined in the manufacture of a medicament for use in therapy.

[0060] The invention further provides a method of effecting immunosuppression (e.g. in the treatment of rheumatoid arthritis, irritable bowel disease, atherosclerosis or psoriasis) which comprises administering a therapeutically effective amount of a compound of formula (I), or a pharmaceutically acceptable salt or solvate thereof, as hereinbefore defined to a patient.

[0061] For the above-mentioned therapeutic uses the dosage administered will, of course, vary with the compound employed, the mode of administration, the treatment desired and the disorder indicated.

[0062] The compounds of formula (I) and pharmaceutically acceptable salts and solvates thereof may be used on their own but will generally be administered in the form of a pharmaceutical composition in which the formula (I) compound/salt/solvate (active ingredient) is in association with a pharmaceutically acceptable adjuvant, diluent or carrier. Depending on the mode of administration, the pharmaceutical composition will preferably comprise from 0.05 to 99% w (per cent by weight), more preferably from 0.10 to 70% w, of active ingredient, and, from 1 to 99.95% w, more preferably from 30 to 99.90% w, of a pharmaceutically acceptable adjuvant, diluent or carrier, all percentages by weight being based on total composition.

[0063] Thus, the present invention also provides a pharmaceutical composition comprising a compound of formula (I), or a pharmaceutically acceptable salt or solvate thereof, as hereinbefore defined in association with a pharmaceutically acceptable adjuvant, diluent or carrier.

[0064] The invention further provides a process for the preparation of a pharmaceutical composition of the invention which comprises mixing a compound of formula (I), or a pharmaceutically acceptable salt or solvate thereof, as hereinbefore defined with a pharmaceutically acceptable adjuvant, diluent or carrier.

[0065] The pharmaceutical composition of the invention may be administered topically (e.g. to the lung and/or airways or to the skin) in the form of solutions, suspensions, heptafluoroalkane aerosols and dry powder formulations; or systemically, e.g. by oral administration in the form of tablets, capsules, syrups, powders or granules, or by parenteral administration in the form of solutions or suspensions, or by subcutaneous administration or by rectal administration in the form of suppositories or transdermally.

[0066] The present invention will now be further illustrated by reference to the following examples.

EXAMPLE 1 2-({3-Nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}carbonyl)benzoic acid

[0067]

[0068] A solution of 1-piperidin-4-yl-1,4-dihydro-2H-3,1-benzoxazin-2-one hydrochloride (J. Med. Chem. 1998, 2157) (0.8 g), 2-(4-chloro-3-nitrobenzoyl)benzoic acid (0.9 g) and triethylamine (0.8 ml) in N,N-dimethylformamide (5 ml) was stirred at room temperature for 72 h. The mixture was partitioned between ethyl acetate and dilute hydrochloric acid, the organic layer was evaporated under reduced pressure. Purification was by chromatography eluting with 4% methanol/dichloromethane. The residue was triturated from methanol, yield 0.4 g as a solid.

[0069] MS: APCI(+ve) 502(M+1)

[0070] 1H NMR: δ(CDCl3/DMSO-d6) 8.13-8.05(2H, m), 7.80(1H, d), 7.70-7.57(2H, m), 7.43-7.33(2H, m), 7.23-7.09(4H, m), 5.12(2H, s), 4.20-4.08(1H, m), 3.55(2H, d), 3.21(2H, t), 2.90-2.80(2H, m), 1.97(2H, d)

[0071] MP:243-4° C.

EXAMPLE 2 1-{1-[2-Nitro-4-(phenylcarbonyl)phenyl]piperidin-4-yl}-1,4-dihydro-2H-3,1-benzoxazin-2-one

[0072]

[0073] The title compound was prepared from 1-piperidin-4-yl-1,4-dihydro-2H-3,1-benzoxazin-2-one hydrochloride (0.3 g) and 4-chloro-3-nitrobenzophenone (0.29 g) using the method of example 1. Yield 0.25 g as a solid.

[0074] MS: APCI(+ve) 458(M+1)

[0075] 1H NMR: δ(CDCl3/DMSO-d6) 8.28(1H, d), 7.98(1H, dd), 7.78-7.75(2H, m), 7.63-7.60(1H, m), 7.53-7.50(2H, m), 7.38(1H, t), 7.22-7.10(4H, m), 5.11(2H, s), 4.25-4.19(1H, m), 3.61(2H, d), 3.23(2H, t), 2.93-2.84(2H, m), 1.98(2H, d)

[0076] MP:272-3° C.

EXAMPLE 3 Methyl 2-({3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}carbonyl)benzoate

[0077]

[0078] The product from example 1 was added to methanolic hydrogen chloride and the mixture stirred overnight. The solvent was removed under reduced pressure and the residue purified by chromatography. Yield 0.03 g.

[0079] MS: APCI(+ve) 516(M+1)

[0080] 1H NMR: δ(CDCl₃) 8.10-8.07(2H, m), 7.91(1H, dd), 7.68-7.57(2H, m), 7.39-7.35(2H, m), 7.19-7.09(4H, m), 5.29(2H, s), 4.22-4.17(1H, m), 3.75(3H, s), 3.57(2H, d), 3.20(2H, t), 2.90-2.81(2H, m), 1.96(2H, d)

[0081] MP: 177-9° C.

EXAMPLE 4 2-({3-Nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}carbonyl)benzamide

[0082]

[0083] A solution of the product from example 1 (0.9 g) and carbonyldiimidazole (1.1 equiv.) in dichloromethane (4 ml) was stirred at room temperature for 1 h, poured onto aqueous ammonia and stirred for a further 1 h. The mixture was extracted with ethyl acetate, the organics washed with water, dried and evaporated under reduced pressure. Purification was by chromatography eluting with 2.5% methanol/dichloromethane. Yield 0.01 g as a solid.

[0084] MS: APCI(+ve) 501(M+1)

[0085] 1H NMR: δ(CDCl₃) 8.08(1H, d), 7.64(1H, d), 7.58-7.34(5H, m), 7.20-7.07(4H, m), 7.03(1H, s), 5.08(2H, s), 4.35(1H, s), 4.21-4.13(1H, m), 3.42(2H, d), 3.04(2H, t), 2.86-2.74(2H, m), 1.90(2H, d)

[0086] MP: 180-2° C.

EXAMPLE 5 Methyl 2-({3-nitro-4-[4-(2-oxo-3,4-dihydroquinolin-1(2H)-yl)piperidin-1-yl]phenyl}carbonyl)benzoate

[0087]

[0088] (i) 2-({3-Nitro-4-[4-(2-oxo-3,4-dihydroquinolin-1(2H)-yl)piperidin-1-yl]phenyl}carbonyl)benzoic acid

[0089] The product was prepared from 1-piperidin-4-yl-3,4-dihydroquinolin-2(1H)-one (Chem. Pharm. Bull. (1996), 44(4), 725-33) (0.45 g) and 2-(4-chloro-3-nitrobenzoyl)benzoic acid (0.6 g) using the method of example 1. Used crude.

[0090] (ii) Methyl 2-({3-nitro-4-[4-(2-oxo-3,4-dihydroquinolin-1(2H)-yl)piperidin-1-yl]phenyl}carbonyl)benzoate

[0091] The title compound was prepared from the product from step (i) (0.2 g) which was added to methanolic hydrogen chloride and stirred at room temperature overnight. The solvent was removed under reduced pressure and the residue partitioned between ethyl acetate and aqueous sodium hydrogencarbonate solution. The organics were separated, dried and evaporated under reduced pressure. Purification was by chromatography to yield 0.18 g of a solid.

[0092] MS: APCI(+ve) 514(M+1)

[0093] 1H NMR: δ(CDCl₃) 8.09-8.07(2H, m), 7.92(1H, dd), 7.68-7.56(2H, m), 7.37(1H, d), 7.26-7.13(4H, m), 7.03(1H, t), 4.50-4.46(1H, m), 3.74(3H, s), 3.53(2H, d), 3.18(2H, t), 2.86-2.75(4H, m), 2.61-2.57(2H, m), 1.84(2H, d)

[0094] MP: 12-3° C.

EXAMPLE 6 2-({3-Nitro-4-[4-(2-oxo-3,4-dihydroquinolin-1(2H)-yl)piperidin-1-yl]phenyl}carbonyl)benzoic acid

[0095]

[0096] Lithium hydroxide hydrate (3 equiv.) was added to a mixture of the product from example 5 step (ii) (0.15 g) in methanol/water (5.5 ml, 10:1) and stirred overnight at room temperature. The solvent was removed under reduced pressure, the residue dissolved in water and neutralised with dilute hydrochloric acid. The mixture was extracted with ethyl acetate, dried and evaporated under reduced pressure. The residue was triturated with ether and the solid collected. Yield 0.06 g.

[0097] MS: APCI(+ve) 500(M+1)

[0098] 1H NMR: δ(CDCl₃) 8.11(2H, m), 7.86(1H, dd), 7.71(1H, m), 7.59(1H, m), 7.38(1H, dd), 7.18(4H, m), 7.01(1H, m), 4.48(1H, m), 3.51(2H, m), 3.16(2H, m), 2.83(4H, m), 2.27(2H, m), 1.84(2H, m)

[0099] MP:201-203° C.

EXAMPLE 7 Methyl 2-({4-[4-(7-chloro-2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-3-nitrophenyl}carbonyl)benzoate

[0100]

[0101] (i) 1,1-Dimethylethyl 4-{[5-chloro-2-(hydroxymethyl)phenyl]amino}piperidine-1-carboxylate

[0102] N-tert-Butoxycarbonyl-4-piperidone (5.8 g), 2-amino-5-chlorobenzyl alcohol (5.02 g) and acetic acid (4 ml) in toluene (200 ml) were heated under reflux using a Dean-Stark trap for 1.5 h. The solvent was evaporated under reduced pressure to ˜100 ml, tetrahydrofuran (100 ml) added followed by sodium cyanoborohydride (6.3 g). Acetic acid (3 ml) was added dropwise to this mixture which was stirred at room temperature for 96 h. The solvents were removed under reduced pressure and the residue partitioned between ethyl acetate and aqueous sodium hydrogencarbonate solution. The organics were dried, evaporated under reduced pressure and the residue triturated with dichloromethane/isohexane. Yield 7.5 g.

[0103] MS: APCI(+ve) 500(M+1)

[0104] (ii) 7-Chloro-1-piperidin-4-yl-1,4-dihydro-2H-3,1-benzoxazin-2-one hydrochloride

[0105] Triphosgene (1.6 g) was added to a stirred solution of the product from step (i) (5 g), N,N-diisopropylethylamine (5.2 ml) in tetrahydrofuran (50 ml) at 0° C. The mixture was stirred at room temperature for 16 h, the precipitate filtered and the filtrate evaporated under reduced pressure. Purification was by chromatography eluting with 20% ethyl acetate/toluene. The product was dissolved in dichloromethane then a solution of hydrogen chloride in 1,4-dioxane added. After 2 h the solvent was removed under reduced pressure to yield a solid. Used directly.

[0106] (iii) Methyl 2-({4-[4-(7-chloro-2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-3-nitrophenyl}carbonyl)benzoate

[0107] Methyl 2-[(4-chloro-3-nitrophenyl)carbonyl]benzoate (0.5 g), the product from step (ii) (0.47 g) and triethylamine (0.5 ml) in N,N-dimethylformamide (2.5 ml) were heated at 60° C. overnight. The mixture was evaporated under reduced pressure and the residue purified by chromatography eluting with 25% ethyl acetate/toluene. Yield 0.7 g of a solid.

[0108] MS: APCI(+ve) 550(M+1)

[0109] 1H NMR: δ(DMSO-d6) 8.00-7.97(2H, m), 7.80-7.72(2H, m), 7.70-7.65(1H, m), 7.46(1H, d), 7.40-7.30(4H, m), 5.12(2H, s), 4.20-4.10(1H, m), 3.64(3H, s), 3.49(2H, br d), 3.26(2H, br t), 2.70-2.60(2H, m), 1.97-1.91(2H, m)

[0110] MP:90-2° C.

EXAMPLES 8-114

[0111] (i) 3-Nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzoic acid

[0112] A solution of 1-piperidin-4-yl-1,4-dihydro-2H-3,1-benzoxazin-2-one hydrochloride (1.0 g), 1,1-dimethylethyl 4-chloro-3-nitrobenzoate (0.95 g) and triethylamine (0.8 g) in N,N-dimethylformamide (10 ml) was stirred at room temperature overnight. The mixture was partitioned between ethyl acetate and water. The organic layer was dried, and evaporated under reduced pressure. Purification was by chromatography eluting with 1:2 ethyl acetate-isohexane. The residue was dissolved in formic acid (5 ml) stirred overnight at room temperature, heated at 55° C. for 2 h, then evaporated under reduced pressure. The residue was triturated with ether, yield 0.85 g as a solid.

[0113] MS: APCI(+ve) 398(M+1)

[0114] (ii) Examples 8-114

[0115] Carbonyldiimidazole (0.2 g) was added to a solution of the product from step (i) (0.4 g) in N,N-dimethylformamide (25 ml) and stirred at room temperature for 2.5 h. The activated acid (0.1 ml) the appropriate amine (5 equivalents) and triethylamine (5 equivalents) in 1-methyl-2-pyrrolidinone (0.1 ml) were left at room temperature for 24 h. The reaction mixture was evaporated to dryness and the residue dissolved in dimethylsulphoxide (0.4 ml).

EXAMPLE 8 N-(1,1-Dimethylethyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0116]

[0117] MS: APCI(+ve) 453(M+1)

EXAMPLE 9 N-[(1R)-2-Hydroxy-1-(phenylmethyl)ethyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0118]

[0119] MS: APCI(+ve) 531(M+1)

EXAMPLE 10 Methyl 2-[({3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}carbonyl)aminolpropanoate

[0120]

[0121] MS: APCI(+ve) 483(M+1)

EXAMPLE 11 3-Nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(tetrahydrofuran-2-ylmethyl)benzamide

[0122]

[0123] MS: APCI(+ve) 481(M+1)

EXAMPLE 12 N-[2-(4-Aminophenyl)ethyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0124]

[0125] MS: APCI(+ve) 516(M+1)

EXAMPLE 13 3-Nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(2,2,2-trifluoroethyl)benzamide

[0126]

[0127] MS: APCI(+ve) 479(M+1)

EXAMPLE 14 Ethyl (2S)-3-methyl-2-[({3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}carbonyl)amino]butanoate

[0128]

[0129] MS: APCI(+ve) 525(M+1)

EXAMPLE 15 Methyl 3-hydroxy-2-[({3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}carbonyl)amino]propanoate

[0130]

[0131] MS: APCI(+ve) 499(M+1)

EXAMPLE 16 N-[2-(3,4-Dihydroxyphenyl)ethyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0132]

[0133] MS: APCI(+ve) 533(M+1)

EXAMPLE 17 3-Nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(2-phenylethyl)benzamide

[0134]

[0135] MS: APCI(+ve) 501(M+1)

EXAMPLE 18 N-[(4-Aminophenyl)methyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0136]

[0137] MS: APCI(+ve) 502(M+1)

EXAMPLE 19 3-Nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(2-thien-2-ylethyl)benzamide

[0138]

[0139] MS: APCI(+ve) 507(M+1)

EXAMPLE 20 N-[3-(Dimethylamino)-2,2-dimethylpropyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0140]

[0141] MS: APCI(+ve) 510(M+1)

EXAMPLE 21 N-{[2,4-Bis(methyloxy)phenyl]methyl}-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0142]

[0143] MS: APCI(+ve) 547(M+1)

EXAMPLE 22 N-Bicyclo[2.2.1]hept-2-yl-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0144]

[0145] MS: APCI(+ve) 491(M+1)

EXAMPLE 23 N-(2-Fluoroethyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0146]

[0147] MS: APCI(+ve) 443 (M+1)

EXAMPLE 24 3-Nitro-N-[(3-nitrophenyl)methyl]-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0148]

[0149] MS: APCI(+ve) 532(M+1)

EXAMPLE 25 N-[(1S,2R)-2-Hydroxy-1-methyl-2-phenylethyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0150]

[0151] MS: APCI(+ve) 531(M+1)

EXAMPLE 26 3-Nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-{[3,4,5-tris(methyloxy)phenyl]methyl}benzamide

[0152]

[0153] MS: APCI(+ve) 577(M+1)

EXAMPLE 27 3-Nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(2-phenylcyclopropyl)benzamide

[0154]

[0155] MS: APCI(+ve) 513(M+1)

EXAMPLE 28 N-[2-Hydroxy-1-(hydroxymethyl)-1-methylethyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamnide

[0156]

[0157] MS: APCI(+ve) 485(M+1)

EXAMPLE 29 N-(1-Azabicyclo[2.2.2]oct-3-yl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0158]

[0159] MS: APCI(+ve) 506(M+1)

EXAMPLE 30 3-Nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(2-piperidin-1-ylethyl)benzamide

[0160]

[0161] MS: APCI(+ve) 508(M+1)

EXAMPLE 31 N-(1,3-Dimethylbutyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0162]

[0163] MS: APCI(+ve) 481(M+1)

EXAMPLE 32 N-(1-Methylbutyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0164]

[0165] MS: APCI(+ve) 467(M+1)

EXAMPLE 33 N-(1-Methylhexyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0166]

[0167] MS: APCI(+ve) 495(M+1)

EXAMPLE 34 N-(3-Methylbutyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0168]

[0169] MS: APCI(+ve) 467(M+1)

EXAMPLE 35 N-[(2-Aminophenyl)methyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0170]

[0171] MS: APCI(+ve) 502(M+1)

EXAMPLE 36 N-[2-Hydroxy-1-(hydroxymethyl)ethyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0172]

[0173] MS: APCI(+ve) 471(M+1)

EXAMPLE 37 N-[2-(Ethylthio)ethyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0174]

[0175] MS: APCI(+ve) 485(M+1)

EXAMPLE 38 N-[(1S)-1-(Hydroxymethyl)-2,2-dimethylpropyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0176]

[0177] MS: APCI(+ve) 497(M+1)

EXAMPLE 39 N-(4-Methylcyclohexyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0178]

[0179] MS: APCI(+ve) 493(M+1)

EXAMPLE 40 N-{2-Hydroxy-1-[(methyloxy)methyl]-2-phenylethyl}-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0180]

[0181] MS: APCI(+ve) 561(M+1)

EXAMPLE 41 N-Ethyl-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0182]

[0183] MS: APCI(+ve) 425(M+1)

EXAMPLE 42 N-Cyclopropyl-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0184]

[0185] MS: APCI(+ve) 437(M+1)

EXAMPLE 43 3-Nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(phenylmethyl)benzamiide

[0186]

[0187] MS: APCI(+ve) 487(M+1)

EXAMPLE 44 N-(1-Methylpropyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0188]

[0189] MS: APCI(+ve) 453(M+1)

EXAMPLE 45 1,1-Dimethylethyl 2-[({3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}carbonyl)anino]ethylcarbamate

[0190]

[0191] MS: APCI(+ve) 440(M+1-Boc)

EXAMPLE 46 N-[2-(3,4-Dihydroxyphenyl)ethyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0192]

[0193] MS: APCI(+ve) 533(M+1)

EXAMPLE 47 N-{[4-(Methyloxy)phenyl]methyl}-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0194]

[0195] MS: APCI(+ve) 517(M+1)

EXAMPLE 48 N-[2-(1H-Imidazol-4-yl)ethyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0196]

[0197] MS: APCI(+ve) 491(M+1)

EXAMPLE 49 N-[(1S)-1-(Hydroxymethyl)propyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0198]

[0199] MS: APCI(+ve) 469(M+1)

EXAMPLE 50 3-Nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-[1-(phenylmethyl)piperidin-4-yl]benzamnide

[0200]

[0201] MS: APCI(+ve) 570(M+1)

EXAMPLE 51 N-[(1R)-1-(Hydroxymethyl)propyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0202]

[0203] MS: APCI(+ve) 469(M+1)

EXAMPLE 52 N-(4-Hydroxybutyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0204]

[0205] MS: APCI(+ve) 469(M+1)

EXAMPLE 53 3-Nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-tricyclo[3.3.1.1˜3,7˜]dec-1-ylbenzamide

[0206]

[0207] MS: APCI(+ve) 531(M+1)

EXAMPLE 54 N-[(1S,2S)-2-Hydroxycyclohexyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0208]

[0209] MS: APCI(+ve) 495(M+1)

EXAMPLE 55 N-(2-Hydroxy-1-methylethyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0210]

[0211] MS: APCI(+ve) 455(M+1)

EXAMPLE 56 N-{2-[(2-Hydroxyethyl)oxy]ethyl}-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0212]

[0213] MS: APCI(+ve) 485(M+1)

EXAMPLE 57 N-[1-(Hydroxymethyl)butyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0214]

[0215] MS: APCI(+ve) 483(M+1)

EXAMPLE 58 N-(2-Amino-2-oxoethyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0216]

[0217] MS: APCI(+ve) 454(M+1)

EXAMPLE 59 N-[1-(4-Fluorophenyl)ethyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0218]

[0219] MS: APCI(+ve) 519(M+1)

EXAMPLE 60 3-Nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(3-phenylpropyl)benzamide

[0220]

[0221] MS: APCI(+ve) 515(M+1)

EXAMPLE 61 N-[(1S,2R)-2-Hydroxycyclohexyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H-yl)piperidin-1-yl]benzamide

[0222]

[0223] MS: APCI(+ve) 495(M+1)

EXAMPLE 62 Ethyl 3-hydroxy-2-[({3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}carbonyl)amino]propanoate

[0224]

[0225] MS: APCI(+ve) 513(M+1)

EXAMPLE 63 N-[(1R,2S)-2-Hydroxy-1-methyl-2-phenylethyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0226]

[0227] MS: APCI(+ve) 531(M+1)

EXAMPLE 64 1-{1-[4-(Morpholin-4-ylcarbonyl)-2-nitrophenyl]piperidin-4-yl}-1,4-dihydro-2H-3,1-benzoxazin-2-one

[0228]

[0229] MS: APCI(+ve) 467(M+1)

EXAMPLE 65 N,N-Dimethyl-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0230]

[0231] MS: APCI(+ve) 425(M+1)

EXAMPLE 66 N,N-Bis(2-hydroxyethyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0232]

[0233] MS: APCI(+ve) 485 (M+1)

EXAMPLE 67 N-(2-Hydroxyethyl)-N-methyl-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0234]

[0235] MS: APCI(+ve) 455(M+1)

EXAMPLE 68 N-(2-Hydroxyethyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(phenylmethyl)benzamide

[0236]

[0237] MS: APCI(+ve) 531 (M+1)

EXAMPLE 69 1-(1-{2-Nitro-4-[(4-phenylpiperazin-1-yl)carbonyl]phenyl}piperidin-4-yl)-1,4-dihydro-2H-3,1-benzoxazin-2-one

[0238]

[0239] MS: APCI(+ve) 542(M+1)

EXAMPLE 70 N-[(1S,2R)-2-hydroxy-1-methyl-2-phenylethyl]-N-methyl-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0240]

[0241] MS: APCI(+ve) 545(M+1)

EXAMPLE 71 N-Ethyl-N-(2-hydroxyethyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0242]

[0243] MS: APCI(+ve) 469(M+1)

EXAMPLE 72 1-[1-(4-{[4-(4-Fluorophenyl)piperazin-1-yl]carbonyl}-2-nitrophenyl)piperidin-4-yl]-1,4-dihydro-2H-3,1-benzoxazin-2-one

[0244]

[0245] MS: APCI(+ve) 560(M+1)

EXAMPLE 73 1-{1-[4-(Azepan-1-ylcarbonyl)-2-nitrophenyl]piperidin-4-yl}-1,4-dihydro-2H-3,1-benzoxazin-2-one

[0246]

[0247] MS: APCI(+ve) 479(M+1)

EXAMPLE 74 N,N-Diethyl-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0248]

[0249] MS: APCI(+ve) 453(M+1)

EXAMPLE 75 N-[2-(Dimethylamino)ethyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(phenylmethyl)benzamide

[0250]

[0251] MS: APCI(+ve) 558(M+1)

EXAMPLE 76 N-Ethyl-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(phenylmethyl)benzamide

[0252]

[0253] MS: APCI(+ve) 515(M+1)

EXAMPLE 77 N-Butyl-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(phenylmethyl)benzamide

[0254]

[0255] MS: APCI(+ve) 543(M+1)

EXAMPLE 78 1-{1-[2-Nitro-4-(piperidin-1-ylcarbonyl)phenyl]piperidin-4-yl}-1,4-dihydro-2H-3,1-benzoxazin-2-one

[0256]

[0257] MS: APCI(+ve) 465(M+1)

EXAMPLE 79 Ethyl [({3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}carbonyl)(phenylmethyl)amino]acetate

[0258]

[0259] MS: APCI(+ve) 573(M+1)

EXAMPLE 80 N-(2-Hydroxyethyl)-N-(1-methylethyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0260]

[0261] MS: APCI(+ve) 483(M+1)

EXAMPLE 81 1-(1-{2-Nitro-4-[(4-pyridin-2-ylpiperazin-1-yl)carbonyl]phenyl}piperidin-4-yl)-1,4-dihydro-2H-3,1-benzoxazin-2-one

[0262]

[0263] MS: APCI(+ve) 543(M+1)

EXAMPLE 82 1-{1-[2-Nitro-4-(pyrrolidin-1-ylcarbonyl)phenyl]piperidin-4-yl}-1,4-dihydro-2H-3,1-benzoxazin-2-one

[0264]

[0265] MS: APCI(+ve) 451(M+1)

EXAMPLE 83 N-(2-Hydroxyethyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-pentylbenzamide

[0266]

[0267] MS: APCI(+ve) 511(M+1)

EXAMPLE 84 N-[2-(Diethylamino)ethyl]-N-ethyl-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0268]

[0269] MS: APCI(+ve) 524(M+1)

EXAMPLE 85 N-Ethyl-N-methyl-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4n)-yl)piperidin-1-yl]benzamide

[0270]

[0271] MS: APCI(+ve) 439(M+1)

EXAMPLE 86 (2S)-1-({3-Nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}carbonyl)pyrrolidine-2-carboxamide

[0272]

[0273] MS: APCI(+ve) 494(M+1)

EXAMPLE 87 N-(2-Cyanoethyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-(phenylmethyl)benzamide

[0274]

[0275] MS: APCI(+ve) 540(M+1)

EXAMPLE 88 1-(1-{4-[(3,5-Dimethylpiperidin-1-yl)carbonyl]-2-nitrophenyl}piperidin-4-yl)-1,4-dihydro-2H-3,1-benzoxazin-2-one

[0276]

[0277] MS: APCI(+ve) 493(M+1)

EXAMPLE 89 1-[1-(4-{[(2R,6S)-2,6-Dimethylmorpholin4-yl]carbonyl}-2-nitrophenyl)piperidin-4-yl]-1,4-dihydro-2H-3,1-benzoxazin-2-one

[0278]

[0279] MS: APCI(+ve) 495(M+1)

EXAMPLE 90 1-{1-[4-({4-[2-(Methyloxy)phenyl]piperazin-1-yl}carbonyl)-2-nitrophenyl]piperidin-4-yl}-1,4-dihydro-2H-3,1-benzoxazin-2-one

[0280]

[0281] MS: APCI(+ve) 572(M+1)

EXAMPLE 91 1-{1-[2-Nitro-4-(thiomorpholin4-ylcarbonyl)phenyl]piperidin-4-yl}-1,4-dihydro-2H-3,1-benzoxazin-2-one

[0282]

[0283] MS: APCI(+ve) 483(M+1)

EXAMPLE 92

[0284]

[0285] MS: APCI(+ve) 548(M+1)

EXAMPLE 93 1-(1-{4-[(4-{2-[(2-Hydroxyethyl)oxy}ethyl}piperazin-1-yl)carbonyl]-2-nitrophenyl}piperidin-4-yl)-1,4-dihydro-2H-3,1-benzoxazin-2-one

[0286]

[0287] MS: APCI(+ve) 554(M+1)

EXAMPLE 94 N-Ethyl-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(pyridin-4-ylmethyl)benzamide

[0288]

[0289] MS: APCI(+ve) 516(M+1)

EXAMPLE 95 N-Methyl-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-prop-2-ynylbenzamide

[0290]

[0291] MS: APCI(+ve) 449(M+1)

EXAMPLE 96 1-(1-{4-[(4-Acetylpiperazin-1-yl)carbonyl]-2-nitrophenyl}piperidin-4-yl)-1,4-dihydro-2H-3,1-benzoxazin-2-one

[0292]

[0293] MS: APCI(+ve) 508(M+1)

EXAMPLE 97 1-[1-(4-{[2-(Hydroxymethyl)piperidin-1-yl]carbonyl}-2-nitrophenyl)piperidin-4-yl]-1,4-dihydro-2H-3,1-benzoxazin-2-one

[0294]

[0295] MS: APCI(+ve) 495(M+1)

EXAMPLE 98 4-({3-Nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}carbonyl)piperazine-1-carbaldehyde

[0296]

[0297] MS: APCI(+ve) 494(M+1)

EXAMPLE 99 N-Methyl-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(phenylmethyl)benzamide

[0298]

[0299] MS: APCI(+ve) 501(M+1)

EXAMPLE 100 Ethyl 4-({3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}carbonyl)piperazine-1-carboxylate

[0300]

[0301] MS: APCI(+ve) 538(M+1)

EXAMPLE 101 Ethyl 1-({3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}carbonyl)piperidine-4-carboxylate

[0302]

[0303] MS: APCI(+ve) 537(M+1)

EXAMPLE 102 1-({3-Nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}carbonyl)piperidine-3-carboxamide

[0304]

[0305] MS: APCI(+ve) 508(M+1)

EXAMPLE 103 1-(1-{4-[(4-Methylpiperazin-1-yl)carbonyl]-2-nitrophenyl}piperidin-4-yl)-1,4-dihydro-2H-3,1-benzoxazin-2-one

[0306]

[0307] MS: APCI(+ve) 480(M+1)

EXAMPLE 104 1-{1-[4-(2,5-Dihydro-1H-pyrrol-1-ylcarbonyl)-2-nitrophenyl]piperidin-4-yl}-1,4-dihydro-2H-3,1-benzoxazin-2-one

[0308]

[0309] MS: APCI(+ve) 449(M+1)

EXAMPLE 105 N-Ethyl-N-(2-methylprop-2-enyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0310]

[0311] MS: APCI(+ve) 479(M+1)

EXAMPLE 106 N,N-Bis(cyanomethyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0312]

[0313] MS: APCI(+ve) 475(M+1)

EXAMPLE 107 N-Butyl-N-(cyanomethyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0314]

[0315] MS: APCI(+ve) 492(M+1)

EXAMPLE 108 N,N-Bis(2-hydroxypropyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H-yl)piperidin-1-yl]benzamide

[0316]

[0317] MS: APCI(+ve) 513(M+1)

EXAMPLE 109 1-(1-{4-[(4-Hydroxypiperidin-1-yl)carbonyl]-2-nitrophenyl}piperidin-4yl)-1,4-dihydro-2H-3,1-benzoxazin-2-one

[0318]

[0319] MS: APCI(+ve) 481(M+1)

EXAMPLE 110 1-(1-{4-[(2,5-Dimethyl-2,5-dihydro-1H-pyrrol-1-yl)carbonyl]-2-nitrophenyl}piperidin-4-yl)-1,4-dihydro-2H-3,1-benzoxazin-2-one

[0320]

[0321] MS: APCI(+ve) 477(M+1)

EXAMPLE 111 N-Methyl-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-propylbenzamide

[0322]

[0323] MS: APCI(+ve) 453(M+1)

EXAMPLE 112 N-(2-Amino-2-oxoethyl)-N-methyl-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0324]

[0325] MS: APCI(+ve) 468(M+1)

EXAMPLE 113 N,N-Diethyl-1-({3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}carbonyl)piperidine-3-carboxamide

[0326]

[0327] MS: APCI(+ve) 564(M+1)

EXAMPLE 114 N-Cyclohexyl-N-methyl-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0328]

[0329] MS: APCI(+ve) 493(M+1)

EXAMPLE 115 N-(1-Methylethyl)-6-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyridazine-3-carboxamide

[0330]

[0331] (i) 6-Chloro-N-(1-methylethyl)pyridazine-3caroxamide

[0332] A solution of 6-chloro-3-pyridazinecarboxylic acid (0.25 g) and carbonyldiimidazole (0.282 g) in N,N-dimethylformamide (10 ml) was stirred at room temperature for 1 h. Isopropylamine (0.162 ml) was added, the mixture stirred for 3 h then partitioned between ethyl acetate and water. The organic layer was washed with water, dried, and evaporated under reduced pressure. Yield 0.284 g.

[0333] 1H NMR: δ(DMSO-d6) 9.02(1H, d), 8.22(1H, d), 8.09(1H, d), 4.22-4.13(1H, m), 1.21(6H, d)

[0334] (ii) N-(1-Methylethyl)-6-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyridazine-3-carboxamide

[0335] 1-Piperidin-4-yl-1,4-dihydro-2H-3,1-benzoxazin-2-one hydrochloride (0.38 g), the product from step (i) (0.28 g) and N,N-diisopropylethylamine (0.73 ml) in I-methyl-2-pyrrolidinone (6 ml) was heated at 100° C. for 8 h. The mixture was partitioned between ethyl acetate and water, the organic layer washed with water, dried, and evaporated under reduced pressure. Purification was by chromatography eluting with 80% ethyl acetate/isohexane to yield 0.225 g of a solid.

[0336] MS: APCI(+ve) 396(M+1)

[0337] 1H NMR: δ(DMSO-d6) 8.51(1H, d), 7.84(1H, d), 7.45-7.35(3H, m), 7.30(1H, d), 7.13(1H, t), 5.14(2H, s), 4.64(2H, br d), 4.31-4.26(1H, m), 4.18-4.09(1H, m), 3.20(2H, t), 2.50-2.44(2H, m), 1.91(2H, br d), 1.19(6H, d)

[0338] MP:120° C.

EXAMPLE 116 N-[2-(Methyloxy)ethyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0339]

[0340] The title compound was prepared from the product of example 8 step (i) and 2-methoxyethylamine (0.5 ml) using the method of example 115 step (i). Yield 0.065 g.

[0341] MS: APCI(+ve) 455(M+1)

[0342] 1H NMR: δ(CDCl₃) 8.22(1H, dd), 7.92(1H, dd), 7.37(1H, t), 7.20-7.09(4H, m), 6.46(1H,br s), 5.10(2H, s), 4.25-4.17(1H, m), 3.68-3.47(6H, m), 3.40(3H, s), 3.15(2H, t), 2.93-2.79(2H, m), 1.95(2H, d)

[0343] MP:192-3° C.

EXAMPLE 117 N-(1-Methylethyl)-6-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyridine-3-carboxamide

[0344]

[0345] (i) 6-Chloro-N-(1-methylethyl)pyridine-3-carboxamide

[0346] The product was prepared from 6-chloro-nicotinic acid (1.0 g), carbonyldiimidazole (0.8 g) and isopropylamine (0.6 ml) using the method of example 115 step (i). Yield 0.75 g.

[0347] MS: APCI(+ve) 199(M+1)

[0348] (ii) N-(1-Methylethyl)-6-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyridine-3-carboxamide

[0349] The title compound was prepared from the product of step (i) (0.4 g) and 1-piperidin-4-yl-1,4-dihydro-2H-3,1-benzoxazin-2-one hydrochloride (0.5 g) using the method of example 115 step (ii). Yield 0.22 g.

[0350] 1H NMR: δ(DMSO-d6) 8.59(1H, d), 7.97-7.94(2H, m), 7.41-7.28(3H, m), 7.12(1H, t), 6.90(1H, d), 5.13(2H, s), 4.56(2H, br d), 4.25-4.18(1H, m), 4.12-4.00(1H, m), 3.06(2H, t), 2.50-2.38(2H, m), 1.85(2H, br d), 1.15(6H, d)

[0351] MP:>230° C.

EXAMPLE 118 5-Chloro-N-(1-methylethyl)-6-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyridine-3-carboxamide

[0352]

[0353] (i) 5,6-Dichloro-N-(1-methylethyl)pyridine-3-carboxamide

[0354] The product was prepared from 5,6-dichloro-nicotinic acid (0.86 g), carbonyldiimidazole (0.8 g) and isopropylamine (0.52 ml) using the method of example 115 step (i). Yield 0.69 g.

[0355] MS: APCI(+ve) 199(M+1)

[0356] (ii) 5-Chloro-N-(1-methylethyl)-6-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyridine-3-carboxamide

[0357] The title compound was prepared from the product of step (i) (0.3 g) and 1-piperidin-4-yl-1,4-dihydro-2H-3,1-benzoxazin-2-one hydrochloride (0.35 g) using the method of example 115 step (ii). Yield 0.187 g.

[0358] MS: APCI(+ve) 429(M+1)

[0359] 1H NMR: δ(DMSO-d6) 8.64(1H, d), 8.25(1H, d), 8.18(1H, d), 7.40(1H, t), 7.33-7.30(2H, m), 7.12(1H, t), 5.15(2H, s), 4.18-4.02(4H, m), 3.06(2H, t), 2.71-2.60(2H, br d), 1.16(6H, d)

[0360] MP:216° C.

EXAMPLE 119 N-(1-Methylethyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzenesulfonamide

[0361]

[0362] (i) 4-Chloro-N-(1-methylethyl)-3-nitrobenzenesulfonamide

[0363] 4-Chloro-3-nitrobenzenesulfonyl chloride (2 g) and isopropylamine (2.1 ml) in dichloromethane (30 ml) was stirred at room temperature for 2 h. The mixture was washed with water, 2M hydrochloric acid, water, dried and evaporated under reduced pressure. Yield 2.2 g.

[0364] 1H NMR: δ(DMSO-d6) 8.45(1H, d), 8.09-7.98(3H, m), 3.39-3.31(1H, septet), 0.99(6H, d)

[0365] (ii) N-(1-Methylethyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzenesulfonamide

[0366] The title compound was prepared from the product of step (i) (0.14 g) and 1-piperidin-4-yl-1,4-dihydro-2H-3,1-benzoxazin-2-one hydrochloride (0.1 g) using the method of example 115 step (ii). Yield 0.037 g.

[0367] MS: APCI(+ve) 475(M+1)

[0368] 1H NMR: δ(DMSO-d6) 8.17(1H, d), 7.86(1H, dd), 7.63(1H, d), 7.48(1H, d), 7.40(1H, t), 7.34-7.30(2H, m), 7.13(1H, t), 5.15(2H, s), 4.19-4.13(1H, m), 3.46(2H, d), 3.32-3.20(3H, m), 2.67-2.59(2H, m), 1.88(2H, d), 0.98(6H, d)

[0369] MP:168° C.

EXAMPLE 120 1-[1-(4-Amino-2-chlorophenyl)piperidin-4-yl]-1,4-dihydro-2H-3,1-benzoxazin-2-one

[0370]

[0371] (i) 1-[1-(2-Chloro-4-nitrophenyl)piperidin-4-yl]-1,4-dihydro-2H-3,1-benzoxazin-2-one

[0372] The product was prepared from 1-piperidin-4yl-1,4-dihydro-2H-3,1-benzoxazin-2-one hydrochloride (1.5 g) and 3-chloro-4-fluoronitrobenzene (1.23 g) using the method of example 115 step (ii). Yield 1.37 g.

[0373] MS: APCI(+ve) 388(M+1)

[0374] (ii) 1-[1-(4-Amino-2-chlorophenyl)piperidin-4-yl]-1,4-dihydro-2H-3,1-benzoxazin-2-one

[0375] Iron powder (1.5 g) was added to a solution of the product from step (i) (1.37 g) in acetic acid (50 ml) and tetrahydrofuran (20 ml). After stirring at room temperature for 5 h, the mixture was filtered through celite, the solvent removed under reduced pressure and the residue partitioned between ethyl acetate and aqueous sodium hydrogencarbonate solution. The organic layer was washed with water, dried and evaporated under reduced pressure. Purification was by chromatography eluting with 50% ethyl acetate/isohexane. Yield 1.05 g.

[0376] MS: APCI(+ve) 358(M+1)

[0377] 1H NMR: δ(DMSO-d6) 7.40(1H, t), 7.29(2H, m), 7.12(1H, t), 6.92(1H, d), 6.64(1H, d), 6.49(1H, dd), 5.14(2H, s), 5.03(2H, s), 3.98-3.92(1H, m), 3.14(2H, d), 2.7-2.62(4H, m), 1.83(2H, br d)

[0378] MP:158° C.

EXAMPLE 121 3-Cyano-N-(1-methylethyl)-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0379]

[0380] (i) Methyl 4-chloro-3-cyanobenzoate

[0381] A solution of sodium nitrite (1.28 g) in water (8 ml) was added over 10 min to a mixture of methyl 3-amino-4-chlorobenzoate (4.0 g) in water (40 ml) and concentrated hydrochloric acid (5 ml) at 0° C. After 30 min the mixture was neutralised with aqueous sodium hydroxide solution to pH˜7 then added portionwise to a solution of copper cyanide (prepared from sodium cyanide (2.87 g) and copper(I) chloride (2.23 g) in water (40 ml)) at 0° C. The mixture was stirred at room temperature for 2 h then partitioned between ethyl acetate and water. The organics were washed with water, dried and evaporated under reduced pressure. The residue was triturated with 20% ethyl acetate/isohexane to yield a solid (1.55 g).

[0382] 1H NMR: δ(CDCl₃) 8.34(1H, d), 8.21-8.17(1H, m), 7.62(1H, dd), 3.96(3H, s)

[0383] (ii) 4-Chloro-3-cyanobenzoic acid

[0384] A solution of the product from step (i) (1.5 g) and lithium hydroxide hydrate (0.84 g) in a mixture of (1:1)water and tetrahydrofuran (40 ml) was stirred at room temperature for 2 h. The tetrahydrofuran was removed under reduced pressure and the residue partitioned between diethyl ether and water. The aqueous layer was acidified with 2M hydrochloric acid then extracted with ethyl acetate. The organic layer was dried and evaporated under reduced pressure. Yield 1.3 g.

[0385] 1H NMR: δ(CDCl₃) 8.42(1H, d), 8.28-8.24(1H, m), 7.67(1H, dd)

[0386] (iii) 4-Chloro-3-cyano-N-(1-methylethyl)benzamide

[0387] The above compound was prepared from the product of step (ii) (0.6 g), carbonyldiimidazole (0.59 g) and isopropylamine (0.51 ml) using the method of example 115 step (i). Yield 0.68 g.

[0388] 1H NMR: δ(CDCl₃) 8.06(1H, d), 7.96-7.92(1H, m), 7.59(1H, d), 5.96(1H, br s), 4.28(1H, septet), 1.29(6H, d)

[0389] (iv) 3-Cyano-N-(1-methylethyl)-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0390] The title compound was prepared from the product of step (iii) (0.29 g) and 1-piperidin-4-yl-1,4-dihydro-2H-3,1-benzoxazin-2-one hydrochloride (0.3 g) using the method of example 115 step (ii). Yield 0.073 g.

[0391] MS: APCI(+ve) 419(M+1)

[0392] 1H NMR: δ(DMSO-d6) 8.21(1H, d), 8.18(1H, d), 8.03(1H, dd), 7.40(1H, t), 7.34-7.30(2H, m), 7.22(1H, d), 7.13(1H, t), 5.16(2H, s), 4.16-4.04(2H, m), 3.81(2H, br d), 3.14(2H, t), 2.75-2.65(2H, m), 1.93(2H, br d), 1.16(6H, d)

[0393] MP:200° C.

EXAMPLE 122 N-{3-Chloro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}-2-methylpropanamide

[0394]

[0395] Isobutyryl chloride (0.017 ml) was added to a stirred solution of the product from example 120 step (ii) (0.05 g) and triethylamine (0.07 ml) in dichloromethane (1 ml) at room temperature. After 2 h the mixture was partitioned between ethyl acetate and water, the organics separated, washed with water, dried, and evaporated under reduced pressure. Trituration with ether gave a solid, yield 0.048 g.

[0396] MS: APCI(+ve) 428(M+1)

[0397] 1H NMR: δ(DMSO-d6) 9.86(1H, s), 7.79(1H, d), 7.46(1H, dd), 7.42-7.38(1H, m), 7.31-7.29(2H, m), 7.14-7.10(2H, m), 5.15(2H, s), 4.04-3.98(1H, m), 3.33-2.84(2H, m), 2.82(2H, t), 2.75-2.65(2H, m), 2.59-2.50(1H, m), 1.87(2H, br d), 1.09(6H, d)

[0398] MP:228° C.

EXAMPLE 123 N-{3-Chloro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}propane-2-sulfonamide

[0399]

[0400] Isopropylsulphonyl chloride (0.03 ml) was added to a stirred solution of the product from example 120 step (ii) (0.05 g), pyridine (0.1 ml) in acetonitrile (0.9 ml) at room temperature. The mixture was stirred overnight, partitioned between ethyl acetate and water, the organics separated, washed with water, dried, and evaporated under reduced pressure. Purification was by chromatography eluting with 40% ethyl acetate/isohexane. Yield 0.015 g.

[0401] MS: APCI(+ve) 464(M+1)

[0402] 1H NMR: δ(DMSO-d6) 9.76(1H, s), 7.40(1H, t), 7.31-7.27(3H, m), 7.19-7.10(3H, m), 5.15(2H, s), 4.04-3.98(1H, m), 3.30(2H, br d), 3.25-3.18(1H, m), 2.82(2H, t), 2.74-2.65(2H, m), 1.87(2H, br d), 1.24(6H, d)

[0403] MP:175° C.

EXAMPLE 124 N-{3-Chloro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}-1-cyanocyclopropanecarboxamide

[0404]

[0405] The title compound was prepared from the product of example 120 step (ii) (0.05 g), carbonyldiimidazole (0.025 g) and 1-cyano-1-cyclopropane carboxylic acid (0.019 g) using the method of example 115 step (i). Yield 0.003 g.

[0406] MS: APCI(+ve) 451 (M+1)

[0407] 1H NMR: δ(DMSO-d6) 10.02(1H, s), 7.70(1H, d), 7.52-7.48(1H, m), 7.40(1H, t), 7.30(2H, t), 7.17-7.10(2H, m), 5.15(2H, s), 4.05-3.99(1H, m), 3.32(2H, d), 2.83(2H, t), 2.74-2.67(2H, m), 1.88(2H, d), 1.67(4H, s)

EXAMPLE 125 (2S)-N-{3-Chloro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}-1-methylpyrrolidine-2-carboxamide

[0408]

[0409] A mixture of the product from example 120 step (ii) (0.1 g), N-methyl-L-proline (0.044 g), N,N-diisopropylethylamine (0.17 ml), 1-hydroxybenzotriazole (0.043 g), 2-(1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium tetrafluoroborate (0.103 g) in N,N-dimethylformamide (3 ml) were stirred at room temperature overnight then partitioned between ethyl acetate and water. The organic layer was washed with water, dried and evaporated under reduced pressure. Purification was by chromatography eluting with 4% methanol/dichloromethane. Yield 0.038 g.

[0410] MS: APCI(+ve) 469(M+1)

[0411] 1H NMR: δ(DMSO-d6) 9.73(1H, s), 7.88(1H, d), 7.59(1H, dd), 7.38(1H, t), 7.30(2H, d), 7.14-7.10(2H, m), 5.15(2H, s), 4.04-3.98(1H, m), 3.30(2H, d), 3.12-3.08(1H, m), 2.90-2.67(5H, m), 2.35-2.29(1H, m), 2.33(3H, s), 2.18-2.09(1H, m) 1.87(2H, br d), 1.82-1.75(3H, m)

[0412] MP: 155° C.

EXAMPLE 126 5-Chloro-N-(1-methylethyl)-6-[4-(4-methyl-2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyridine-3-carboxamide

[0413]

[0414] (i) 1,1-Dimethylethyl 4-(4-methyl-2-oxo-2H-3,1-benzoxazin-1(4H)-yi)piperidine-1-carboxylate

[0415] Acetic acid (1 ml) was added dropwise to a solution of N-tert-butoxycarbonyl-4-piperidone (9.4 g), 1-(2-amino-phenyl)-ethanol (4.3 g) and sodium cyanoborohydride (10 g) in dichloromethane and the mixture stirred at room temperature overnight. The mixture was partitioned between ethyl acetate and water, the organics separated and washed with aqueous sodium hydrogencarbonate solution, water, dried, and evaporated under reduced pressure. The crude product was dissolved in tetrahydrofuran (100 ml) and N,N-diisopropylethylamine (23 ml), cooled to 0° C., then triphosgene (4.3 g) added. The mixture was warmed to room temperature and stirred overnight. The mixture was partitioned between ethyl acetate and water, the organics washed with water, dried and evaporated under reduced pressure. Purification was by chromatography eluting with 20% ethyl acetate/isohexane. Yield 1.4 g.

[0416] MS: APCI(+ve) 247(M+1-Boc)

[0417] (ii) 4-Methyl-1-piperidin-4-yl-1,4-dihydro-2H-3,1-benzoxazin-2-one hydrochloride

[0418] 4M Hydrogen chloride in 1,4-dioxane (20 ml) was added to a solution of the product from step (i) (1.4 g) in 1,4-dioxane (20 ml) and the mixture stirred at room temperature overnight. The solvent was removed under reduced pressure and the residue triturated with ether. Yield 1.0 g.

[0419] MS: APCI(+ve) 247(M+1)

[0420] (iii) 5-Chloro-N-(1-methylethyl)-6-[4-(4-methyl-2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyridine-3-carboxamide

[0421] The title compound was prepared from the product of step (ii) (0.36 g) and the product from example 117 step (i) (0.466 g) using the method of example 115 step (ii). Yield 0.112 g

[0422] MS: APCI(+ve) 443(M+1)

[0423] 1H NMR: δ(DMSO-d6) 8.64(1H, d), 8.25(1H, d), 8.18(1H, d), 7.42-7.38(1H, m), 7.34-7.27(2H, m), 7.16-7.12(1H, m), 5.36(1H, q), 4.18-4.05(4H, m), 3.09-3.02(2H, m), 2.72-2.61(2H, m), 1.89(2H, br d), 1.57(3H,d), 1.16(6H, d)

EXAMPLES 127 ±-5-Chloro-N-(1-methylethyl)-6-[(cis)-3-methyl-4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyridine-3-carboxamide hydrochloride

[0424]

EXAMPLE 128 ±-5-Chloro-N-(1-methylethyl)-6-[(trans)-3-methyl-4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyridine-3-carboxamide hydrochloride

[0425]

[0426] (i) 1,1-Dimethylethyl 4-{[2-(hydroxymethyl)phenyl]amino}-3-methylpiperidine-1-carboxylate

[0427] The product was prepared from N-tert-butoxycarbonyl-3-methyl-4-piperidone (4.3 g) and 2-amino-benzyl alcohol (2.59 g) using the method of example 7 step (i). Yield 6.3 g as a mixture of diastereoisomers.

[0428] MS: APCI(+ve) 320(M+1)

[0429] (ii) ±-1,1-Dimethylethyl (cis)-3-methyl-4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidine-1-carboxylate

[0430] ±-1,1-Dimethylethyl (trans)-3-methyl-4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidine-1-carboxylate

[0431] The above compounds were prepared from the product of step (i) (6.3 g) using the method of example 7 step (ii). Cis and trans diastereoisomers were separated (relative stereochemistry).

[0432] ±-1,1-Dimethylethyl (cis)-3-methyl-4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidine-1-carboxylate, yield 0.24 g MS: APCI(+ve) 247(M+1)

[0433] ±-1,1-Dimethylethyl (trans)-3-methyl-4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidine-1-carboxylate, yield 0.68 g MS: APCI(+ve) 247(M+1)

[0434] (iii) ±-5-Chloro-N-(1-methylethyl)-6-1(cis)-3-methyl-4-(2-oxo-2-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyridine-3-carboxaniide hydrochloride

[0435] ±-1,1-Dimethylethyl (cis)-3-methyl-4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidine-1-carboxylate (0.24 g) was dissolved in 4M hydrogen chloride in 1,4-dioxane (5 ml) stirred at room temperature for 4 h, then evaporated under reduced pressure. The product was dissolved in 1-methyl 2-pyrrolidinone (10 ml), N,N-diisopropylethylamine (0.5 ml) and the product from example 117 step (i) (0.23 g) added. The mixture was heated at 100° C. for 12 h, partitioned between ethyl acetate and water, the organics separated, dried and evaporated under reduced pressure. Purification was by chromatography eluting with 30-40% ethyl acetate/isohexane. The hydrochloride salt was made from ethereal hydrogen chloride. Yield 0.07 g.

[0436] MS: APCI(+ve) 443(M+1)

[0437] 1H NMR: δ(DMSO-d6) 8.64(1H, d), 8.27(1H, d), 8.19(1H, d), 7.41-7.30(3H, m), 7.12(1H, t), 6.12(2H, br s), 5.22-5.14(2H, m), 4.12-3.99(3H, m), 3.77(1H, br s), 3.13(1H, br t), 2.86(1H, br s), 2.75-2.50(2H, m), 1.94(1H, br d), 1.16(6H, d), 0.87(3H, d)

[0438] MP:215° C.

[0439] ±-5-chloro-N-(1-methylethyl)-6-(trans)-3-methyl-4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyridine-3-carboxamide hydrochloride

[0440] The titled compound was prepared from ±-1,1-dimethylethyl (trans)-3-methyl-4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidine-1-carboxylate (0.68 g) using the same method as step (iii). Yield 0.219 g.

[0441] MS: APCI(+ve) 443(M+1)

[0442] 1H NMR: δ(DMSO-d6) 8.63(1H, s), 8.25(1H, d), 8.17(1H, s), 7.40-7.29(2H, m), 7.28(1H, d), 7.12(1H, t), 6.04(2H, br s), 5.21-5.13(2H, m), 4.29-4.24(1H, m), 4.12-3.91(3H, m), 3.30(1H, dd), 3.18-3.02(2H, m), 2.56-2.54(1H, m), 1.87(1H, br d), 1.16(6H, d), 1.09(3H, d)

[0443] MP:195° C.

EXAMPLES 129-144

[0444] (i) 2-[4-(2-Oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-4-(trifluoromethyl)pyrimidine-5-carboxylic acid

[0445] The title compound was prepared from 1-piperidin-4-yl-1,4-dihydro-2H-3,1-benzoxazin-2-one hydrochloride (0.70 g) and 2-chloro-4-(trifluoromethyl)pyrimidine-5-carboxylic acid (1.8 g) using the method of example 115 step (ii). Yield 1.1 g.

[0446] MS: APCI(+ve) 423(M+1)

[0447] (ii) Examples 129-144

[0448] Oxalyl chloride (0.1 ul) was added to a solution of the product from step (i) (0.27 g) in dichloromethane (10 ml) and stirred at room temperature for 3 h. The solvent was removed under reduced pressure and the residue dissolved in 1-methyl-2-pyrrolidinone. An aliquot of the solution of the acid chloride (0.1 ml), the appropriate amine (2 equivalents) and triethylamine (5 equivalents) in 1-methyl-2-pyrrolidinone (0.03 ml) were left at room temperature for 24 h. The reaction mixture was evaporated to dryness and the residue dissolved in dimethylsulphoxide (0.4 ml).

EXAMPLE 129 2-[4-(2-Oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(1,3,4-thiadiazol-2-yl)-4-(trifluoromethyl)pyrimidine-5-carboxamide

[0449]

[0450] MS: APCI(+ve) 505(M+1)

EXAMPLE 130 2-[4-(2-Oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(1H-1,2,4-triazol-3-yl)-4-(trifluoromethyl)pyrimidine-5-carboxamide

[0451]

[0452] MS: APCI(+ve) 488(M+1)

EXAMPLE 131 2-[4-(2-Oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(1H-pyrazol-3-yl)-4-(trifluoromethyl)pyrimidine-5-carboxamide

[0453]

[0454] MS: APCI(+ve) 487(M+1)

EXAMPLE 132 N-(4-Hydroxycyclohexyl)-2-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-4-(trifluoromethyl)pyrimidine-5-carboxamide

[0455]

[0456] MS: APCI(+ve) 519(M+1)

EXAMPLE 133 N-[1-(Hydroxymethyl)propyl]-2-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-4-(trifluoromethyl)pyrimidine-5-carboxamide

[0457]

[0458] MS: APCI(+ve) 493(M+1)

EXAMPLE 134 N-(3-Hydroxy-2,2-dimethylpropyl)-2-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-4-(trifluoromethyl)pyrimidine-5-carboxamide

[0459]

[0460] MS: APCI(+ve) 507 (M+1)

EXAMPLE 135 2-[4-(2-Oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(tetrahydrofuran-2-ylmethyl)-4-(trifluoromethyl)pyrimidine-5-carboxamide

[0461]

[0462] MS: APCI(+ve) 505(M+1)

EXAMPLE 136 N-Cyclobutyl-2-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-4-(trifluoromethyl)pyrimidine-5-carboxamiide

[0463]

[0464] MS: APCI(+ve) 475(M+1)

EXAMPLE 137 N-Cyclopentyl-2-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-4-(trifluoromethyl)pyrimidine-5-carboxamide

[0465]

[0466] MS: APCI(+ve) 489(M+1)

EXAMPLE 138 N-[2-(1H-Imidazol-4-yl)ethyl]-2-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-4-(trifluoromethyl)pyrimidine-5-carboxamide

[0467]

[0468] MS: APCI(+ve) 515(M+1)

EXAMPLE 139 N-(1-Ethynylcyclohexyl)-2-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-4-(trifluoromethyl)pyrimdine-5-carboxamide

[0469]

[0470] MS: APCI(+ve) 527(M+1)

EXAMPLE 140 N-[(1R)-1-(Hydroxymethyl)-2-methylpropyl]-2-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-4-(trifluoromethyl)pyrimidine-5-carboxamide

[0471]

[0472] MS: APCI(+ve) 507(M+1)

EXAMPLE 141 N-(2-Hydroxy-1,1-dimethylethyl)-2-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-4-(trifluoromethyl)pyrimidine-5-carboxamide

[0473]

[0474] MS: APCI(+ve) 493(M+1)

EXAMPLE 142 N-(1,1-Diethylprop-2-ynyl)-2-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-4-(trifluoromethyl)pyrimidine-5-carboxamide

[0475]

[0476] MS: APCI(+ve) 515(M+1)

EXAMPLE 143 N-(2-Hydroxy-1-methylethyl)-2-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-4-(trifluoromethyl)pyrimidine-5-carboxamide

[0477]

[0478] MS: APCI(+ve) 479(M+1)

EXAMPLE 144 N-[1-Methyl-2-(methyloxy)ethyl]-2-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-4-(trifluoromethyl)pyrimidine-5-carboxamide

[0479]

[0480] MS: APCI(+ve) 493(M+1)

EXAMPLE 145 N-(1-Methylethyl)-2-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyrimidine-5-carboxaamide

[0481]

[0482] (i) N-(1-Methylethyl)-2-(methylthio)pyrimidine-5-carboxamide

[0483] The product was prepared from N-(1-methylethyl)-2-(methylthio)pyrimidine-5-carboxylic acid (Acta Chem Scand., Ser.B (1986), B40(9), 764-767.) (0.78 g), carbonyldiimidazole (0.82 g) and isopropylaniine (0.3 g) using the method of example 115 step (i). Yield 0.66 g.

[0484] MS: APCI(+ve) 212 (M+1)

[0485] (ii) N-(1-Methylethyl)-2-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyrimidine-5-carboxamide

[0486] The product from step (i) (0.66 g) was dissolved in chloroform (50 ml) and to this solution was added 3-chloroperoxybenzoic acid (2.02 g). The mixture was stirred for 1 h at room temperature before being washed with an aqueous solution of sodium metabisulphite followed by aqueous sodium bicarbonate. The organic layer was dried and evaporated under reduced pressure. The residue was dissolved in 1-methyl-2-pyrrolidinone (4 ml) and this solution treated with N,N-diisopropylethylamine (0.5 ml) followed by 1-piperidin-4-yl-1,4-dihydro-2H-3,1-benzoxazin-2-one hydrochloride (0.2 g) before being heated at 60° C. for 2 h. The mixture was partitioned between water and ethyl acetate, the organic layer washed with water, dried and evaporated under reduced pressure. Purification was by chromatography eluting with ethyl acetate/isohexane (2/1). Yield 0.03 g as a solid.

[0487] MS: APCI(+ve) 396 (M+1)

[0488] 1H NMR: δ(DMSO-d6) 8.77(2H, s), 8.07(1H, d), 7.41-7.29(3H, m), 7.12(1H, t), 5.14(2H, s), 4.88(2H, d), 4.28-4.22(1H, m), 4.11-4.02(1H, m), 3.12(2H, t), 2.45-2.3(2H, m), 1.89(2H, d), 1.16(6H, d)

[0489] MP:236-239° C.

EXAMPLE 146 N-(1-Methylethyl)-2-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-1,3-thiazole4-carboxamide

[0490]

[0491] (i) 2-Bromo-N-(1-methylethyl)-1,3-thiazole4-carboxamide

[0492] The product was prepared from 2-bromo-N-(1-methylethyl)-1,3-thiazole-4-carboxylic acid (WO 9848799) (0.77 g), carbonyldiimidazole (0.66 g) and isopropylamine (0.24 g) using the method of example 115 step (i). Yield 0.82 g.

[0493] 1H NMR: δ(DMSO-d6) 8.25(1H, s), 8.24-8.18(1H, m), 4.14-4.02(1H, m), 1.16(6H, d)

[0494] (ii) N-(1-Methylethyl)-2-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-1,3-thiazole4-carboxamide

[0495] The title compound was prepared from the product of step (i) (0.16 g) and 1-piperidin-4-yl-1,4-dihydro-2H-3,1-benzoxazin-2-one hydrochloride (0.15 g) using the method of example 115 step (ii). Yield 0.04 g.

[0496] MS: APCI(+ve) 401 (M+1)

[0497] 1H NMR: δ(DMSO-d6) 7.68(1H, d), 7.43-7.29(4H, m), 7.13(1H, t), 5.16(2H, s), 4.22-3.99(4H, m), 3.29-3.18(2H, m), 2.64-2.49(2H, m), 1.91(2H, d), 1.16(6H, d)

[0498] MP:214-215° C.

EXAMPLE 147 N-(1-Methylethyl)-3-(methylsulfonyl)-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0499] 4-Fluoro-N-(1-methylethyl)-3-(methylsulfonyl)benzoic acid (J.Med.Chem (1997), 40(13), 2017-2034) (0.45 g) was reacted with carbonyldiimidazole (0.37 g) and isopropylamine (0.25 g) using the method of example 115 step (i) to yield the corresponding amide. Yield 0.50 g.

[0500] A solution of this amide (0.50 g) in 1-methyl-2-pyrrolidinone (10 ml) was treated with 1-piperidin-4-yl-1,4-dihydro-2H-3,1-benzoxazin-2-one hydrochloride (0.40 g) followed by N,N-diisopropylethylamine (0.73 g) and the resultant mixture heated at 100° C. for 14 h. The mixture was then partitioned between water and ethyl acetate, the organic layer washed with water, dried and evaporated under reduced pressure. The resultant solid was washed with ethyl acetate (10 ml) followed by ethanol (1 ml) to yield the desired product as a solid (0.13 g).

[0501] MS: APCI(+ve) 472 (M+1)

[0502] 1H NMR: δ(DMSO-d6) 8.47(1H, d), 8.38(1H, d), 8.16(1H, d), 7.63(1H, d), 7.42(1H, t), 7.32-7.29(2H, m), 7.13(1H, t), 5.16(2H, s), 4.14-4.05(2H, m), 3.49(3H, s), 3.32-3.29(2H, m), 3.03(2H, t), 2.83-2.76(2H, m), 1.91-1.88(2H, m), 1.17(6H, d)

[0503] MP:240-242° C.

EXAMPLE 148 N-[(1R)-1-(Aminocarbonyl)-2-methylpropyl]-5-chloro-6-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyridine-3-carboxamide

[0504]

[0505] (i) 5-Chloro-6-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyridine-3-carboxylic acid

[0506] The title compound was prepared from 5,6-dichloronicotinic acid (2.2 g) and 1-piperidin-4-yl-1,4-dihydro-2H-3,1-benzoxazin-2-one hydrochloride (3.0 g) using the method of example 115 step (ii). Yield 0.037 g

[0507] MS: APCI(+ve) 388 (M+1)

[0508] (ii) N-[(1R)-1-(Aminocarbonyl)-2-methylpropyl]-5-chloro-6-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyridine-3-carboxamide

[0509] The product of step (i) (0.14 g) was dissolved in 1-methyl-2-pyrrolidinone (4 ml) and to this solution was added carbonyldiimidazole (0.064 g) the mixture was stirred at room temperature for 1 h and then treated with D-valinamide hydrochloride (0.11 g) and N,N-diisopropylethylamine (0.10 g). After stirring for 18 h at room temperature the mixture was partitioned between aqueous sodium bicarbonate and ethyl acetate, the organic layer was washed with water, dried and evaporated under reduced pressure. The resultant solid was washed with ethyl acetate to yield 0.06 g of product.

[0510] MS: APCI(+ve) 486 (M+1)

[0511] 1H NMR: δ(DMSO-d6) 8.68(1H, d), 8.31-8.27(2H, m), 7.46(1H, s), 7.42-7.29(3H, m), 7.13(1H, t), 7.06(1H, s), 5.15(2H, s), 4.26(1H, t), 4.19-4.04(3H, m), 3.07(2H, t), 2.72-2.60(2H, m), 2.14-2.07(1H, m), 1.90(2H, d), 0.94-0.91(6H, m)

[0512] MP:140-143° C.

EXAMPLE 149 5-Chloro-N-(2-hydroxy-1-methylethyl)-6-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyridine-3-carboxamide

[0513]

[0514] The title compound was prepared from the product of example 148 step (i) (0.14 g), carbonyldiimidazole (0.064 g) and DL-2-amino-1-propanol (0.05 g) using the method of example 115 step (i). Purification was by chromatography eluting with 20% ethyl acetate/isohexane. Yield 0.04 g as a solid.

[0515] MS: APCI(+ve) 445 (M+1)

[0516] 1H NMR: δ(DMSO-d6) 8.64(1H, d), 8.20(1H, d), 8.15(1H, d), 7.42-7.29(3H, m), 7.12(1H, t), 5.15(2H, s), 4.72(1H, t), 4.15-3.98(4H, m), 3.48-3.37(2H, m), 3.06(2H, t), 2.73-2.62(2H, m), 1.89(2H, d), 1.13-1.09(3H, m)

[0517] MP:125-128° C.

EXAMPLE 150 5-Chloro-N-(1,1-dimethylprop-2-ynyl)-6-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyridine-3-carboxamide

[0518]

[0519] The title compound was prepared from the product of example 148 step (i) (0.14 g), carbonyldiimidazole (0.064 g) and 1,1-dimethylpropargylamine (0.06 g) using the method of example 115 step (i). Purification was by chromatography eluting with ethyl acetate/isohexane (2/3). Yield 0.03 g as a solid.

[0520] MS: APCI(+ve) 453 (M+1)

[0521] 1H NMR: δ(DMSO-d6) 8.62(1H, d), 8.29(1H, s), 8.18(1H, d), 7.42-7.29(3H, m), 7.12(1H, t), 5.15(2H, s), 4.19-4.07(3H, m), 3.12(1H, s), 3.07(2H, t), 2.72-2.60(2H, m), 1.90(2H, d), 1.60(6H, s)

[0522] MP:135-138° C.

EXAMPLE 151 N-(2-Amino-1-cyano-2-oxoethyl)-5-chloro-6-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyridine-3-carboxamide

[0523]

[0524] The product of example 148 step (i) (0.14 g) was stirred as a suspension in dichloromethane (4 ml) and to this mixture was added oxalyl chloride (0.05 g) followed by N,N-dimethylformamide (0.01 g). After stirring for 1 h at room temperature the mixture was treated with 2-aminocyanoacetanide (0.14 g) 1-methyl-2-pyrrolidinone (3 ml) and then N,N-diisopropylethylamine (1 ml), stirring was then continued for a further 18 h at room temperature. The reaction mixture was partitioned between aqueous sodium bicarbonate and ethyl acetate, the organic layer was washed with water, dried and evaporated under reduced pressure. Purification was by chromatography eluting with 25% ethyl acetate/isohexane. Yield 0.09 g as a solid.

[0525] MS: APCI(+ve) 469 (M+1)

[0526] 1H NMR: δ(DMSO-d6) 9.63(1H, d), 8.69(1H, d), 8.25(1H, d), 7.83(1H, s), 7.68(1H, s), 7.40(1H, t), 7.34-7.30(2H, m), 7.13(1H, t), 5.67(1H, d), 5.15(2H, s), 4.17-4.14(3H, m), 3.10(2H, t), 2.69-2.61(2H, m), 1.91(2H, d)

[0527] MP: 159-162° C.

EXAMPLE 152 N-[(1R)-1-(Aminocarbonyl)-3-methylbutyl]-5-chloro-6-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyridine-3-carboxamide

[0528]

[0529] The title compound was prepared from the product of example 148 step (i) (0.15 g) and (R)-leucinamide hydrochloride (0.07 g) according to the method of example 115, step (i). Yield 0.05 g as a solid.

[0530] MS: APCI(+ve) 500 (M+1)

[0531] 1H NMR: δ(DMSO-d6) 8.68(1H, d), 8.45(1H, d), 8.27(1H, d), 7.42-7.29(4H, m), 7.12(1H, t), 6.98(1H, s), 5.15(2H, s), 4.44-4.41(1H, m), 4.15-4.07(3H, m), 3.07(2H, t), 2.68-2.64(2H, m), 1.90(2H, d), 1.70-1.54(3H, m), 0.92-0.86(6H, m)

[0532] MP:139-142° C.

EXAMPLE 153 N-[(1S)-1-(Amiocarbonyl)-2-methylpropyl]-5-chloro-6-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyridine-3-carboxamide

[0533]

[0534] The title compound was prepared from the product of example 148 step (i) (0.15 g) and (S)-valinamide hydrochloride (0.08 g) according to the method of example 115, step (i). Yield 0.03 g as a solid.

[0535] MS: APCI(+ve) 486 (M+1)

[0536] 1H NMR: δ(DMSO-d6) 8.68(1H, t), 8.31-8.28(2H, m), 7.46-7.29(4H, m), 7.13(1H, t), 7.06(1H, s), 5.15(2H, s), 4.24-4.00(4H, m), 3.07(2H, t), 2.73-2.61(2H, m), 2.14-2.05(1H, m), 1.90(2H, d), 0.94(3H, s), 0.92(3H, s)

EXAMPLE 154 N-[(1S)-1-(Aminocarbonyl)-3-methylbutyl]-5-chloro-6-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyridine-3-carboxamide

[0537]

[0538] The title compound was prepared from the product of example 148 step (i) (0.15 g) and (S)-leucinamide hydrochloride (0.07 g) according to the method of example 115, step (i). Yield 0.04 g as a solid.

[0539] MS: APCI(+ve) 500 (M+1)

[0540] 1H NMR: δ(DMSO-d6) 8.68(1H, d), 8.45(1H, d), 8.27(1H, d), 7.42-7.29(4H, m), 7.13(1H, t), 6.98(1H, s), 5.15(2H, s), 4.44-4.42(1H, m), 4.15-4.04(3H, m), 3.07(2H, t), 2.68-2.64(2H, m), 1.90(2H, d), 1.66-1.54(3H, m), 0.92-0.86(6H, m)

[0541] MP: 139-142° C.

EXAMPLE 155 5-Chloro-N-(1-methylethyl)-6-[4-(3-oxo-2,3-dihydro-4H-1,4-benzoxazin-4-yl)piperidin-1-yl]pyridine-3-carboxamide

[0542]

[0543] The title compound was prepared from 4-piperidin-4-yl-4H-benzo[1,4]oxazin-3-one hydrochloride (WO 9502405) (0.13 g) and the product from example 117 step (i) (0.13 g) according to the method of example 115 step (ii). Yield 0.04 g as a solid.

[0544] MS: APCI(+ve) 429 (M+1)

[0545] 1H NMR: δ(DMSO-d6) 8.64(1H, d), 8.25(1H, d), 8.18(1H, d), 7.40(1H, d), 7.12-7.03(3H, m), 4.52(2H, s), 4.37-4.32(1H, m), 4.11-4.04(3H, m), 3.03(2H, t), 2.77-2.6(2H, m), 1.82(2H, d), 1.16(6H, d)

[0546] MP:85-88° C.

EXAMPLE 156 N-(1-Methylethyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0547]

[0548] The product from example 8 step (i) (0.05 g) was reacted with isopropylamine (0.02 ml) using the method of example 115 step (i) in N,N-dimethylformamide (2 ml). Purification was by chromatography eluting with (2:1) ethyl acetate/isohexane. Yield 0.035 g as a solid.

[0549] MS: APCI(+ve) 439(M+1)

[0550] 1H NMR: δ(CDCl₃) 8.17(1H, m), 7.93-7.90(1H, m), 7.38-7.09(5H, m), 5.92-5.90(1H, d), 5.10(2H, s), 4.32-4.16(2H, m), 3.53-3.49(2H, m), 3.17-3.10(2H, m), 2.90-2.80(2H, m), 1.96-1.93(2H, m), 1.28-1.26(6H, d)

[0551] MP: 193-195° C.

EXAMPLE 157 N-[(1S)-1-(Aminocarbonyl)-2-methylbutyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0552]

[0553] The product from example 8 step (i) (0.05 g) was reacted with (S)-leucinamide hydrochloride (0.025 g) using the method of example 115 step (i) in N,N-dimethylformamide (2 ml). Purification was by chromatography eluting with ethyl acetate. Yield 0.025 g as a solid.

[0554] MS: APCI(+ve) 510(M+1)

[0555] 1H NMR: δ(CDCl₃) 8.29-7.09(6H, m), 6.90(1H, d), 6.22(1H, br s), 5.56(1H, br s), 5.10(2H, s), 4.73-4.11(2H, m), 3.50-2.80(6H, m), 1.97-1.70(5H, m), 1.27-1.22(1H, m), 0.99(6H, d)

[0556] MP: 146-149° C.

EXAMPLE 158 N-(1-Methylethyl)-2-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-4-(trifluoromethyl)-5-pyrimidine-5-carboxamide.

[0557]

[0558] (i) N-(1-Methylethyl)-2-chloro-4-(trifluoromethyl)pyriniidine-5-carboxamide

[0559] 2-Chloro-4-(trifluoromethyl)pyrimidine-5-carbonyl chloride (1.0 g) in dry N,N-dimethylformamide (5 ml) was treated with isopropylamine (0.4 ml) at 0° C. The reaction mixture was stirred at 0° C. for 30 min, diluted with water, extracted with ethyl acetate, dried, and evaporated under reduced pressure. Purification was by chromatography eluting with 50% ethyl acetate/dichloromethane. Yield 0.74 g as a solid.

[0560] MS: APCI(+ve) 268(M+1)

[0561] (ii) N-(1-Methylethyl)-2-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl]piperidin-1-yl]-4-(trifluoromethyl)-5-pyrimidine-5-carboxamide.

[0562] The title compound was prepared from the product from step (i) (0.092 g) and 1-piperidin-4-yl-1,4-dihydro-2H-3,1-benzoxazin-2-one hydrochloride (0.1 g) using the method of example 115 step(ii). Purification was by chromatography eluting with (1:3) ethyl acetate/dichloromethane. Yield 0.110 g as a solid.

[0563] MS: APCI(+ve) 464(M+1)

[0564] 1H NMR: δ(CDCl₃) 8.58(1H, s), 7.38-7.08(4H, m), 5.62-5.60(1H, d), 5.10-5.05(4H, m), 4.29-4.15(2H, m), 3.06-2.62(4H, m), 1.99-1.95(2H, d), 1.25-1.24(6H, d)

[0565] MP: 217-219° C.

EXAMPLE 159 3-Chloro-N-(1-methylethyl)-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0566]

[0567] (i) 1,1-Dimethylethyl 3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzoate

[0568] The product was prepared from 3-nitro-4-chloro-t-butylbenzoate (0.96 g) and 1-piperidin-4-yl-1,4-dihydro-2H-3,1-benzoxazin-2-one hydrochloride (1.0 g) using the method of example 115 step (ii). Purification was by chromatography eluting with 50% ethyl acetate/isohexane. Yield 2.1 g as an oil.

[0569] MS: APCI(+ve) 454(M+1)

[0570] (ii) 3-Amino-1,1-dimethylethyl-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzoate

[0571] The product from step (i) (1.9 g) was dissolved in glacial acetic acid (20 ml) and treated with reduced iron powder (1.9 g). The mixture was stirred vigorously for 2 h at room temperature. The mixture was filtered through a pad of celite and the filtrate evaporated under reduced pressure. Purification was by chromatography eluting with (1:5) ethyl acetate/ dichloromethane. Yield 0.975 g as a solid.

[0572] MS: APCI(+ve) 424(M+1)

[0573] (iii) 3-Chloro-N-(1-methylethyl)-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H )-yl)piperidin-1-yl]benzamide

[0574] Product from step (ii) (0.39 g) was treated with copper(II) chloride (0.148 g), isoamylnitrite (0.25 ml) in acetonitrile (10 ml) and heated to 65° C. for 4 h. The reaction mixture was evaporated under reduced pressure to an oil. The oil was treated with trifluoroacetic acid/dichloromethane (1:1) and stirred at room temperature for 2 h then evaporated under reduced pressure. The residue was dissolved in N,N-dimethylformnamide (5 ml), bromo-tris-pyyrolidino-phosphonium hexafluorophosphate (0.116 g), isopropylamine (0.054 ml) and N,N-diisopropylethylamine (0.06 ml) were added and stirred at room temperature for 16 h. The mixture was evaporated under reduced pressure. Purification was by chromatography eluting with (1:3) ethyl acetate/dichloromethane. Yield 0.017 g as a solid.

[0575] MS: APCI(+ve) 428(M+1)

[0576] 1H NMR: δ(CDCl₃) 7.76-7.06(7H, m), 5.82-5.80(1H, d), 5.10(2H, s), 4.31-4.15(2H, m), 3.65-3.62(2H, m), 2.95-2.82(4H, m), 1.98-1.95(2H, d), 1.27-1.25(6H, d).

EXAMPLE 160 3-Amino-N-(1-methylethyl)-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0577]

[0578] The title compound was prepared from the product of example 156 using the method described in example 159 step (ii). Yield 0.6 g as a solid.

[0579] MS: APCI(+ve) 409(M+1)

[0580] 1H NMR: δ(DMSO-d6) 7.89-7.86(1H, d), 7.39-6.91(7H, m), 5.15(2H, s), 4.84-4.82(2H, s), 4.08-3.99(2H, m), 3.23-3.21(2H, m), 2.77-2.67(4H, m), 1.88-1.85(2H, m), 1.19-1.12(6H, d)

EXAMPLE 161 N-(1-Methylethyl)-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide,

[0581]

[0582] (i) 1,1-Dimethylethyl 4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzoate

[0583] A solution of sodium nitrite (0.11 g) in water (1 ml) was added to a stirred solution of the product from example 159 step (ii) (0.456 g) in acetonitrile (10 ml) at room temperature. After 1 h a solution of iron sulphate (0.3 g) in N,N-dimethylformamide (20 ml) was added and the mixture stirred for a further 30 min. The mixture was partitioned between ethyl acetate and water, the organics dried and evaporated under reduced pressure. Purification was by chromatography eluting with 20% ethyl acetate/isohexane. Yield 0.29 g as an oil.

[0584] MS: APCI(+ve) 409(M+1)

[0585] (ii) N-(1-Methylethyl)-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0586] A solution of the product from step (i) (0.29 g) in a mixture of trifluoroacetic acid (10 ml) and dichloromethane (10 ml) was stirred at room temperature for 1 h. The solution was evaporated under reduced pressure, the residue dissolved in N,N-dimethylformamide then bromo-tris-pyyrolidino-phosphonium hexafluorophosphate (0.16 g), isopropylamine (0.06 ml) and N,N-diisopropylethylamine (0.06 ml) added. The solution was stirred at room temperature for 16 h then evaporated under reduced pressure. Purification was by chromatography eluting with (1:5) ethyl acetate/dichloromethane. Yield 0.01 g as a solid.

[0587] MS: APCI(+ve) 394(M+1)

[0588] 1H NMR: δ(DMSO-d6) 7.89-6.95(9H, m), 5.13(2H, s), 4.15-3.97(4H, m), 3.01-2.95(2H, m), 2.59-2.49(2H, m), 1.85-1.82(2H, d), 1.13(6H, d)

EXAMPLE 162 N-[(1S)-1-(Aminocarbonyl)-3-methylbutyl]-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0589]

[0590] The title compound was prepared from the product of example 161 step (i) (0.06 g) and (S)-leucinamide hydrochloride (0.056 g) using the method of example 161 step (ii). Purification was by chromatography eluting with (5:1) ethyl acetate/dichloromethane. Yield 0.01 g as a solid.

[0591] MS: APCI(+ve) 465(M+1)

[0592] 1H NMR: δ(DMSO-d6) 8.04-6.92(11H, m), 5.13(2H, s), 4.44-3.99(4H, m), 3.03-2.93(2H, m), 2.60-2.49(2H, m), 1.85-1.50(5H, m), 0.88(6H, d)

EXAMPLE 163 3-(Ethylamino)-N-(1-methylethyl)-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0593]

[0594] Sodium triacetoxyborohydride (0.1 g) was added to a solution of the product from example 160 (0.1 g), acetaldehyde (0.015 ml), acetic acid (1 drop) in N,N-dimethylformamide (10 ml). The reaction mixture was stirred at room temperature for 16 h. The mixture was diluted with water, extracted with ethyl acetate, dried, and evaporated under reduced pressure. Yield 0.035 g.

[0595] MS: APCI(+ve) 437(M+1)

[0596] 1H NMR: δ(DMSO-d6) 7.96-6.98(8H, m), 5.15(2H, s), 4.73-4.70(1H, t), 4.13-3.99(2H, m), 3.25-3.14(4H, m), 2.81-2.64(4H, m), 1.90-1.88(2H, m), 1.26-1.14(9H, m)

EXAMPLE 164 3-(Diethylamino)-N-(1-methylethyl)-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0597]

[0598] The title compound was obtained from the reaction mixture in example 163. Yield 0.052 g.

[0599] MS: APCI(+ve) 465(M+1)

[0600] 1H NMR: δ(DMSO-d6) 7.97-6.90(8H, m), 5.15(2H, s), 4.11-3.99(2H, m), 3.86-3.83(2H, m), 3.31-3.18(4H, m), 2.72-2.63(4H, m), 1.86(2H, m), 1.19-1.14(6H, d), 1.03-0.95(6H, m)

EXAMPLE 165 N-(1-Methylethyl)-3-[(methylsulfonyl)amino]-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide

[0601]

[0602] The title compound was prepared from the product of example 160 (0.1 g) and methanesulphonylchloride (0.02 ml) in dichloromethane (10 ml) at 0° C. in the presence of 2,6-lutidine (0.085 ml). The reaction mixture was stirred at room temperature for 16 h. The mixture was evaporated, dissolved in ethyl acetate, washed with water, dried, and evaporated under reduced pressure. Purification was by chromatography eluting with (3:1) ethyl acetate/isohexane. Yield 0.066 g as a solid.

[0603] MS: APCI(+ve) 487(M+1)

[0604] 1H NMR: δ(DMSO-d6) 8.63(1H,s), 8.16-8.13(1H, d), 7.76-7.10(7H, m), 5.16(2H, s), 4.14-3.99(2H, m), 3.27-3.23(2H, m), 3.19(3H, s), 2.93-2.70(4H, m), 1.89-1.86(2H, m), 1.17-1.14(6H, d)

EXAMPLE 166 5-Chloro-N-(1-methylethyl)-6-[4-(2-oxo-1,3-benzoxazol-3(2H)-yl)piperidin-1-yl]pyridine-3-carboxantide

[0605]

[0606] (i) 1,1-Dimethylethyl 4-(2-oxo-1,3-benzoxazol-3(2H)-yl)piperidine-1-carboxylate

[0607] 2-Benzoxazolinone (1 g) was added to a cooled solution of triphenylphoshine (2.13 g) and diethylazodicarboxylate (1.28 ml) in dry tetrahydrofuran (20 ml). After 10 min at 0° C., N-(t-butoxy)-4-hydroxypiperidine (1.63 g) (Tetrahedron Letters, 1996, 6439-6442) was added portionwise. The reaction mixture was stirred at room temperature for 16 h. The solution was diluted with water, extracted with ethyl acetate, dried and evaporated under reduced pressure. Purification was by chromatography eluting with (1:2) diethylether/isohexane. Yield 0.5 g as an oil.

[0608] MS: APCI(+ve) 219(M+1)-BOC

[0609] (ii) 3-Piperidin-4-yl-1,3-benzoxazol-2(3H)-one, trifluoroacetic acid salt

[0610] The product from step (i) (0.5 g) was stirred at room temperature in (1:1) trifluoroacetic acid/dichloromethane (10 ml) for 30 min. The reaction mixture was evaporated under reduced pressure to give an oil. Used crude.

[0611] MS: APCI(+ve) 219(M+1)

[0612] (iii) 5-Chloro-N-(1-methylethyl)-6-[4-(2-oxo-1,3-benzoxazol-3(2H)-yl)piperidin-1-yl]pyridine-3-carboxamide

[0613] The title compound was prepared from the product of example 117 step(i) (0.53 g) and the product from step (ii) by the method described in example 115 step (ii). Purification was by chromatography eluting with 50% ethyl acetate/isohexane. Yield 0.28 g as a solid.

[0614] MS: APCI(+ve) 415(M+1)

[0615] 1H NMR: δ(DMSO-d6) 8.66-7.12(7H, m), 4.44-4.38(1H, m), 4.10-4.05(3H, m), 3.09-3.03(2H, t), 2.45-2.35(2H, m), 1.95-1.92(2H, m), 1.17-1.15(6H, d)

[0616] MP: 162-168° C.

EXAMPLES 167-169

[0617] (i) 4-Chloro-N-(1-methylethyl)-3-nitrobenzamide

[0618] Isopropylamine (1.28 ml) was added dropwise to a stirred solution of 4-chloro-3-nitrobenzoylchloride (3.0 g) and triethylamine (2.8 ml) in dichloromethane (30 ml) at room temperature. After 2 h the mixture was partitioned between ethyl acetate and water, the organics dried and evaporated under reduced pressure. Yield 2.87 g.

[0619] 1H NMR: δ(DMSO-d6) 8.58(1H, d), 8.51(1H, d), 8.15(1H, dd), 7.89(1H, d), 4.14-4.06(1H, m), 1.18(6H, d)

[0620] (ii) Examples 167-169

[0621] A solution of the product from step (i) (1 mg), N,N-diisopropylethylamine (3 equiv.), the appropriate amine (1.5 equiv.) in 1-methyl-2-pyrrolidinone (0.16 ml) were heated at 65° C. for 30 h. The reaction mixture was evaporated to dryness and the residue dissolved in dimethylsulphoxide (0.4 ml).

EXAMPLE 167 N-(1-Methylethyl)-3-nitro-4-[4-(2-oxo-3,4-dihydroquinolin-1(2H)-yl)piperidin-1-yl]benzamide

[0622]

[0623] MS: APCI(+ve) 436(M+1)

EXAMPLE 168 4-[4-(7-Chloro-2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(1-methylethyl-3-nitrobenzamide

[0624]

[0625] MS: APCI(+ve) 472(M+1)

EXAMPLE 169 5-Chloro-N-(1-methylethyl)-6-[4-(2-oxo-3,4-dihydroquinolin-1(2H)-yl)piperidin-1-yl]pyridine-3-arboxamide

[0626]

[0627] The title compound was prepared from 1-piperidin-4-yl-3,4-dihydro-1H-quinolin-2-one (0.03 g) and the product from example 117 step (i) (0.03 g) by the method of example 115 step (ii). Purification was by chromatography eluting with 50% ethyl acetate/I-hexane. Yield 0.017 g as a white solid.

[0628] MS: APCI(+ve) 427 (M+1)

[0629] 1H NMR: δ(DMSO-d6) 8.49(1H, d), 7.98(1H, d), 7.20(3H, m), 7.02(1H, t), 5.83(1H, d), 4.50(1H, m), 4.20(3H, m), 2.98(2H, t), 2.80(4H, m), 2.60(2H, t) 1.83(2H, m), 1.25(6H, d)

EXAMPLE 170 4-[4-(2,2-Dioxido-3,4-dihydro-1H-2,1,3-benzothiadiazin-1-yl)piperidin-1-yl]-N-(1-methylethyl)-3-nitrobenzamide

[0630]

[0631] The title compound was prepared from 1-Piperidin-4-yl-3,4-dihydro-1H-benzo[1,2,6]thiadiazine 2,2-dioxide (Chem. Pharm. Bull. (1985), 33(3), 1104-15) (0.05 g) and the product from example 167 step (i) (0.05 g) by the method of example 115 step (ii). Purification was by chromatography eluting with ethyl acetate. Yield 0.03 g as a white solid.

[0632] MS: APCI(+ve) 474 (M+1)

[0633] 1H NMR: δ(DMSO-d6) 8.32 (2H, m), 8.00 (1H, d), 7.72 (1H, t), 7.30 (2H, m), 7.20 (2H, d), 7.10 (1H, t), 4.41(2H, d), 4.10 (3H, m), 3.40 (1H, m), 3.04 (2H, t), 2.00 (4H, m), 1.15 (6H,d).

EXAMPLE 171 1-(1-{2-Chloro-4-[(1H-imidazol-2-ylmethyl)amino]phenyl}piperidin-4-yl)-1,4-dihydro-2H-3,1-benzoxazin-2-one

[0634]

[0635] The product of example 120 step (ii) (0.25 g) was dissolved in 1-methyl-2-pyrrolidinone (6 ml) and this solution was treated with 2-imidazolecarboxaldehyde (0.1 g) followed by acetic acid (0.13 g) and then sodium triacetoxyborohydride (0.37 g). the reaction mixture was stirred at room temperature for three days. At the end of this time the mixture was poured in to excess aqueous dilute hydrochloric acid, this solution was allowed to stand for 10 minutes before being basified by addition of excess aqueous sodium bicarbonate. The mixture was extracted with ethyl acetate, the organic layer was washed with water, dried and evaporated under reduced pressure. Purification was by chromatography eluting with methanol/chloroform (7/93). Yield 0.05 g as a solid.

[0636] MS: APCl (=ve) 438 (M+1)

[0637] 1H NMR: δ(DMSO-d6) 11.84 (1H, s), 7.41 (1H, t), 7.31-7.27(2H, m), 7.11(1H, t), 6.97(1H, d), 6.92(2H, s), 6.73(1H, d), 6.57(1H, q), 6.06(1H, t), 5.14(2H, s), 4.19(2H, d), 3.98-3.93(1H, m), 3.15(2H, d), 2.78-2.63(4H, m) 1.83(2H, d)

[0638] MP:222-224° C.

[0639] Pharmacological Analysis

[0640] Certain compounds such as benzoylbenzoyl adenosine triphosphate (bbATP) are known to be agonists of the P2X₇ receptor, effecting the formation of pores in the plasma membrane (Drug Development Research (1996), 37(3), p.126). Consequently, when the receptor is activated using bbATP in the presence of ethidium bromide (a fluorescent DNA probe), an increase in the fluorescence of intracellular DNA-bound ethidium bromide is observed. The increase in fluorescence can be used as a measure of P2X₇ receptor activation and therefore to quantify the effect of a compound on the P2X₇ receptor.

[0641] In this manner, each of the title compounds was tested for antagonist activity at the P2X₇ receptor. Thus, the test was performed in 96-well flat bottomed microtitre plates, the wells being filled with 100 μl of test solution comprising 80 μl of a suspension of THP-1 cells (2.5×10⁶ cells/ml) containing 10⁻⁴M ethidium bromide, 10 μl of a high potassium buffer solution containing 10⁻⁵M bbATP, and 10 μl of the high potassium buffer solution containing 1×10⁻⁴M test compound (in 10% v/v DMSO). The plate was covered with a plastic lid and incubated at 37° C. for one hour. The plate was then read in a Spectromax Gemini Fluorescent plate reader excitation 525 nm, emission 610 nm, slit widths: Ex 15 nm, Em 20 nm. For the purposes of comparison, bbATP (a P2X₇ receptor agonist) and N-(5-methoxy-2-methylphenyl)-tricyclo[3.3.1.1^(3,7)]decane-1-acetamide (WO99/29660, a P2X₇ receptor antagonist) were used separately in the test as controls. From the readings obtained, a pIC₅₀ figure was calculated for each test compound, this figure being the negative logarithm of the concentration of test compound necessary to reduce the bbATP agonist activity by 50%. The pIC₅₀ was then corrected using a modified Cheng Prusoff calculation based on agonist A₅₀ (Trends in Pharmacological Sciences (1993), 14(4), 110-2). Each of the compounds of the Examples demonstrated antagonist activity, having a pIC₅₀ figure>5.00. 

1. A compound of formula (I):

where A is phenyl or a 5- or 6-membered heterocyclic ring containing one or two heteroatoms selected from O, N or S; and optionally substituted by C₁₋₆alkyl, halogen, nitro, amino, alkylamino, CF₃, SO₂Me, NHSO₂Me or cyano; B is C═O, NH or SO₂; X is C═O, CH(Me), O or (CH₂)p where p is 0 or 1; Y is O, CH₂, NH or S; Z is C═O or SO₂, provided that when Z is C═O, then Y is O, CH₂ or S; R is hydrogen or C₁₋₆alkyl; R¹ is hydrogen, halogen; R² is phenyl optionally substituted by CO₂H, CO₂alkyl, CONH₂ or R² is OH, NHR³, NHCH(R⁴)(CHR⁵)_(n)R⁶, NH—R⁷—R⁸, SO₂NHalkyl, NHCOalkyl, NHSO₂alkyl, morpholine, NR⁹R¹⁰, piperazine substituted by phenyl, alkoxyphenyl, pyridyl or fluorophenyl; n is 0, 1 or 2; R³ is hydrogen, a bi- or tricyclic saturated ring system optionally containing a nitrogen atom, piperidinyl, alkylpyrollidine, ethynylcyclohexyl, a 5-membered aromatic ring containing 2 or 3 heteroatoms, C₄₋₆ cycloalkyl optionally substituted by alkyl, cyano or hydroxy, or C₁₋₈ alkyl optionally containing an oxygen atom in the alkyl chain and being optionally substituted by one or more substituents selected from ethynyl, cyano, fluoro, di-alkylamino, hydroxy, thioalkyl, CO₂R¹¹ or CONH₂; R⁴ is hydrogen or alkyl optionally substituted by hydroxy or alkoxy; R⁵ is hydrogen or hydroxy; R⁶ is CO₂R¹¹, NHCO₂R¹², CONH₂ or a 5 or 6-membered saturated ring containing an oxygen atom, a 5-membered heterocyclic ring containing one or two heteroatoms selected from O, N or S, or phenyl optionally substituted by one or more groups selected from alkyl, hydroxy, amino, alkoxy, or nitro; R⁶ is alkyl; R⁷ is a cyclopentane ring; R⁸ is phenyl; R⁹ and R¹⁰ are independently hydrogen, benzyl, alkenyl, cycloalkyl, alkyl optionally substituted by hydroxy, alkoxy, cyano, dialkylamino, phenyl, pyridyl or CO₂R¹¹ or R⁹ and R¹⁰ together form a 5- to 7-membered saturated or partially saturated ring optionally containing a further heteroaton and optionally substituted by one or more groups selected from alkyl (optionally containing an oxygen atom in the chain and optionally substituted by hydroxy), COalkyl, CO₂R¹¹, COR¹³R¹⁴, CHO or piperidine, R¹¹ is hydrogen or alkyl; R² is alkyl; and R¹³ and R¹⁴ are independently hydrogen or alkyl, or a pharmaceutically acceptable salt or solvate thereof.
 2. A compound according to claim 1 in which A is phenyl optionally substituted by C₁₋₆alkyl, halogen, nitro, amino, alkylamino, CF₃, SO₂Me, NHSO₂Me or cyano.
 3. A compound according to claim 1 or 2 in which B is C═O.
 4. A compound according to any one of claims 1 to 3 in which X is CH₂, Y is O and Z is C═O.
 5. A compound according to any one of claims 1 to 4 in which R is hydrogen.
 6. A compound according to any one of claims 1 to 5 in which R¹ is hydrogen.
 7. A compound according to any one of claims 1 to 6 in which R² is NR⁹R¹⁰ where one of R⁹ or R¹⁰ is hydrogen and the other is alkyl.
 8. A compound according to claim 1 which is: 2-({3-Nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}carbonyl)benzoic acid, 1-{1-[2-Nitro-4-(phenylcarbonyl)phenyl]piperidin-4-yl}-1,4-dihydro-2H-3,1-benzoxazin-2-one, Methyl 2-({3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}carbonyl)benzoate, 2-({3-Nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}carbonyl)benzamide, Methyl 2-({3-nitro-4-[4-(2-oxo-3,4-dihydroquinolin-1(2H)-yl)piperidin-1-yl]phenyl}carbonyl)benzoate, 2-({3-Nitro-4-[4-(2-oxo-3,4-dihydroquinolin-1(2H)-yl)piperidin-1-yl]phenyl}carbonyl)benzoic acid, Methyl 2-({4-[4-(7-chloro-2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-3-nitrophenyl}carbonyl)benzoate, N-(1,1-Dimethylethyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-[(1R)-2-Hydroxy-1-(phenylmethyl)ethyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, Methyl 2-[({3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}carbonyl)amino]propanoate, 3-Nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(tetrahydrofuran-2-ylmethyl)benzamide, N-[2-(4-Aminophenyl)ethyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, 3-Nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(2,2,2-trifluoroethyl)benzamide, Ethyl (2S)-3-methyl-2-[({3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}carbonyl)amino]butanoate, Methyl 3-hydroxy-2-[({3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}carbonyl)amino]propanoate, N-[2-(3,4-Dihydroxyphenyl)ethyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, 3-Nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(2-phenylethyl)benzamide, N-[(4-Aminophenyl)methyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, 3-Nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(2-thien-2-yl-ethyl)benzamide, N-[3-(Dimethylamino)-2,2-dimethylpropyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-{[2,4-Bis(methyloxy)phenyl]methyl}-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-Bicyclo[2.2.1]hept-2-yl-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-(2-Fluoroethyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, 3-Nitro-N-[(3-nitrophenyl)methyl]-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-[(1S,2R)-2-Hydroxy-1-methyl-2-phenylethyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, 3-Nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-{[3,4,5-tris(methyloxy)phenyl]methyl}benzamide, 3-Nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(2-phenylcyclopropyl)benzamide, N-[2-Hydroxy-1-(hydroxymethyl)-1-methylethyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-(1-Azabicyclo[2.2.2]oct-3-yl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, 3-Nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(2-piperidin-1-ylethyl)benzamide, N-(1,3-Dimethylbutyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-(1-Methylbutyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-(1-Methylhexyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-(3-Methylbutyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-[(2-Aminophenyl)methyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-[2-Hydroxy-1-(hydroxymethyl)ethyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl)piperidin-1-yl]benzamide, N-[2-(Ethylthio)ethyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-[(1S)-1-(Hydroxymethyl)-2,2-dimethylpropyl]-3-nitro-4-[4-(2-oxo-2H-3,1-1(4H)-yl)piperidin-1-yl]benzamide, N-(4-Methylcyclohexyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-{2-Hydroxy-1-[(methyloxy)methyl]-2-phenylethyl}-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-Ethyl-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide N-Cyclopropyl-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, 3-Nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(phenylmethyl)benzamide, N-(1-Methylpropyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, 1,1-Dimethylethyl 2-[({3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}carbonyl)amino]ethylcarbamate, N-[2-(3,4-Dihydroxyphenyl)ethyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-{[4-(Methyloxy)phenyl]methyl}-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-[2-(1H-Imidazol-4-yl)ethyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-[(1S)-1-(Hydroxymethyl)propyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, 3-Nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-[1-(phenylmethyl)piperidin-4-yl]benzamide, N-[(1R)-1-(Hydroxymethyl)propyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-(4-Hydroxybutyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, 3-Nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-tricyclo[3.3.1.1˜3,7˜]dec-1-ylbenzamide, N-[(1S,2S)-2-Hydroxycyclohexyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-(2-Hydroxy-1-methylethyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-{2-[(2-Hydroxyethyl)oxy]ethyl}-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-yl)piperidin-1-yl]benzamide, N-[1-(Hydroxymethyl)butyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-(2-Amino-2-oxoethyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-[1-(4-Fluorophenyl)ethyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, 3-Nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(3-phenylpropyl)benzamide, N-[(1S,2R)-2-Hydroxycyclohexyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, Ethyl 3-hydroxy-2-[({3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}carbonyl)amino]propanoate, N-[(1R,2S)-2-Hydroxy-1-methyl-2-phenylethyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, 1-{1-[4-(Morpholin-4-ylcarbonyl)-2-nitrophenyl]piperidin-4-yl}-1,4-dihydro-2H-3,1-benzoxazin-2-one, N,N-Dimethyl-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N,N-Bis(2-hydroxyethyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-(2-Hydroxyethyl)-N-methyl-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-1-yl]benzamide, N-(2-Hydroxyethyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(phenylmethyl)benzamide, 1-(1-{2-Nitro-4-[(4-phenylpiperazin-1-yl)carbonyl]phenyl}piperidin-4-yl)-1,4-dihydro-2H-3,1-benzoxazin-2-one, N-[(1S,2R)-2-hydroxy-1-methyl-2-phenylethyl]-N-methyl-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-Ethyl-N-(2-hydroxyethyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, 1-[1-(4-{[4-(4-Fluorophenyl)piperazin-1-yl]carbonyl}-2-nitrophenyl)piperidin-4-yl]-1,4-dihydro-2H-3,1-benzoxazin-2-one, 1-{1-[4-(Azepan-1-ylcarbonyl)-2-nitrophenyl]piperidin-4-yl}-1,4-dihydro-2H-3,1-benzoxazin-2-one, N,N-Diethyl-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-[2-(Dimethylamino)ethyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(phenylmethyl)benzamide, N-Ethyl-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(phenylmethyl)benzamide, N-Butyl-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(phenylnethyl)benzamide, 1-{1-[2-Nitro-4-(piperidin-1-ylcarbonyl)phenyl]piperidin-4-yl}-1,4-dihydro-2H-3,1-benzoxazin-2-one, Ethyl [({3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}carbonyl)(phenylmethyl)amino]acetate, N-(2-Hydroxyethyl)-N-(1-methylethyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, 1-(1-{2-Nitro-4-[(4-pyridin-2-ylpiperazin-1-yl)carbonyl]phenyl}piperidin-4-yl)-1,4-dihydro-2H-3,1-benzoxazin-2-one, 1-{1-[2-Nitro-4-(pyrrolidin-1-ylcarbonyl)phenyl]piperidin-4-yl}-1,4-dihydro-2H-3,1-benzoxazin-2-one, N-(2-Hydroxyethyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-pentylbenzamide, N-[2-(Diethylamino)ethyl]-N-ethyl-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-Ethyl-N-methyl-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, (2S)-1-({3-Nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}carbonyl)pyrrolidine-2-carboxamide, N-(2-Cyanoethyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(phenylmethyl)benzamide, 1-(1-{4-[(3,5-Dimethylpiperidin-1-yl)carbonyl]-2-nitrophenyl}piperidin-4-yl)-1,4-dihydro-2H-3,1-benzoxazin-2-one, 1-[1-(4-{[(2R,6S)-2,6-Dimethylmorpholin-4-yl]carbonyl}-2-nitrophenyl)piperidin-4-yl]-1,4-dihydro-2H-3,1-benzoxazin-2-one, 1-{1-[4-({4-[2-(Methyloxy)phenyl]piperazin-1-yl}carbonyl)-2-nitrophenyl]piperidin-4-yl}-1,4-dihydro-2H-3,1-benzoxazin-2-one, 1-{1-[2-Nitro-4-(thiomorpholin-4-ylcarbonyl)phenyl]piperidin-4yl}-1,4-dihydro-2H-3,1-benzoxazin-2-one, 1-(1-{4-[(4-{2-[(2-Hydroxyethyl)oxy]ethyl}piperazin-1-yl)carbonyl]-2-nitrophenyl}piperidin-4-yl)-1,4-dihydro-2H-3,1-benzoxazin-2-one, N-Ethyl-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(pyridin-4-ylmethyl)benzamide, N-Methyl-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-prop-2-ynylbenzamide, 1-(1-{4-[(4-Acetylpiperazin-1-yl)carbonyl]-2-nitropheny}piperidin-4-yl)-1,4-dihydro-2H-3,1-benzoxazin-2-one, 1-[1-(4-{[2-(Hydroxymethyl)piperidin-1-yl]carbonyl}-2-nitrophenyl)piperidin-4-yl]-1,4-dihydro-2H-3,1-benzoxazin-2-one, 4-({3-Nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}carbonyl)piperazine-1-carbaldehyde, N-Methyl-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(phenylmethyl)benzamide, Ethyl 4-({3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}carbonyl)piperazine-1-carboxylate, Ethyl 1-({3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}carbonyl)piperidine-4-carboxylate, 1-({3-Nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}carbonyl)piperidine-3-carboxamide, 1-(1-{4-[(4-Methylpiperazin-1-yl)carbonyl]-2-nitrophenyl}piperidin-4-yl)-1,4-dihydro-2H-3,1-benzoxazin-2-one, 1-{1-[4-(2,5-Dihydro-1H-pyrrol-1-ylcarbonyl)-2-nitrophenyl]piperidin-4-yl}-1,4-dihydro-2H-3,1-benzoxazin-2-one, N-Ethyl-N-(2-methylprop-2-enyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N,N-Bis(cyanomethyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-Butyl-N-(cyanomethyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N,N-Bis(2-hydroxypropyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, 1-(1-{4-[(4-Hydroxypiperidin-1-yl)carbonyl]-2-nitrophenyl}piperidin-4-yl)-1,4-dihydro-2H-3,1-benzoxazin-2-one, 1-(1-{4-[(2,5-Dimethyl-2,5-dihydro-1H-pyrrol-1-yl)carbonyl]-2-nitrophenyl}piperidin-4-yl)-1,4-dihydro-2H-3,1-benzoxazin-2-one, N-Methyl-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-propylbenzamnide, N-(2-Amino-2-oxoethyl)-N-methyl-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N,N-Diethyl-1-({3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}carbonyl)piperidine-3-carboxamide, N-Cyclohexyl-N-methyl-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-[2-(Methyloxy)ethyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-(1-Methylethyl)-6-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyridine-3-carboxamide, 5-Chloro-N-(1-methylethyl)-6-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyridine-3-carboxamide, N-(1-Methylethyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzenesulfonamide, 1-[1-(4-Amino-2-chlorophenyl)piperidin-4-yl]-1,4-dihydro-2H-3,1-benzoxazin-2-one, 3-Cyano-N-(1-methylethyl)-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-{3-Chloro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}-2-methylpropanamide, N-{3-Chloro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}propane-2-sulfonamide, N-{3-Chloro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}-1-cyanocyclopropanecarboxamide, (2S)-N-{3-Chloro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]phenyl}-1-methylpyrrolidine-2-carboxamide, 5-Chloro-N-(1-methylethyl)-6-[4-(4-methyl-2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyridine-3-carboxamide, ±-5-Chloro-N-(1-methylethyl)-6-[(cis)-3-methyl-4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyridine-3-carboxamide, ±-5-Chloro-N-(1-methylethyl)-6-[(trans)-3-methyl-4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyridine-3-carboxamide, 2-[4-(2-Oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(1,3,4-thiadiazol-2-yl)-4-(trifluoromethyl)pyrimidine-5-carboxamide, 2-[4-(2-Oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(1H-1,2,4-triazol-3-yl)-4-(trifluoromethyl)pyrimidine-5-carboxamide, 2-[4-(2-Oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(1H-pyrazol-3-yl)-4-(trifluoromethyl)pyrimidine-5-carboxamide, N-(4-Hydroxycyclohexyl)-2-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-4-(trifluoromethyl)pyrimidine-5-carboxamide, N-[1-(Hydroxymethyl)propyl]-2-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-4-(trifluoromethyl)pyrimidine-5-carboxamide, N-(3-Hydroxy-2,2-dimethylpropyl)-2-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-4-(trifluoromethyl)pyrimidine-5-carboxamide, 2-[4-(2-Oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(tetrahydrofuran-2-ylmethyl)-4-(trifluoromethyl)pyrimidine-5-carboxamide, N-Cyclobutyl-2-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-4-(trifluoromethyl)pyrimidine-5-carboxamide, N-Cyclopentyl-2-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-4-(trifluoromethyl)pyrimidine-5-carboxamide, N-[2-(1H-Imidazol-4-yl)ethyl]-2-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-4-(trifluoromethyl)pyrimidine-5-carboxamide, N-(1-Ethynylcyclohexyl)-2-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-4-(trifluoromethyl)pyrimidine-5-carboxamide, N-[(1R)-1-(Hydroxymethyl)-2-methylpropyl]-2-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-4-(trifluoromethyl)pyrimidine-5-carboxamide, N-(2-Hydroxy-1,1-dimethylethyl)-2-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-4-(trifluoromethyl)pyrimidine-5-carboxamnide, N-(1,1-Diethylprop-2-ynyl)-2-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-4-(trifluoromethyl)pyrimidine-5-carboxamide, N-(2-Hydroxy-1-methylethyl)-2-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-4-(trifluoromethyl)pyrimidine-5-carboxamide, N-[1-Methyl-2-(methyloxy)ethyl]-2-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-4-(trifluoromethyl)pyrimidine-5-carboxamide, N-(1-Methylethyl)-2-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyrimidine-5-carboxamide, N-(1-Methylethyl)-2-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-1,3-thiazole-4-carboxamide, N-(1-Methylethyl)-3-(methylsulfonyl)-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-[(1R)-1-(Aminocarbonyl)-2-methylpropyl]-5-chloro-6-[4-(2-oxo-2H-3,1-benzoxzin-1(4H)-yl)piperidin-1-yl]pyridine-3-carboxamide, 5-Chloro-N-(2-hydroxy-1-methylethyl)-6-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyridine-3-carboxamide, 5-Chloro-N-(1,1-dimethylprop-2-ynyl)-6-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyridine-3-carboxamide, N-(2-Amino-1-cyano-2-oxoethyl)-5-chloro-6-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyridine-3-carboxamide, N-[(1R)-1-(Aminocarbonyl)-3-methylbutyl]-5-chloro-6-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyridine-3-carboxamide, N-[(1S)-1-(Aminocarbonyl)-2-methylpropyl]-5-chloro-6-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyridine-3-carboxamide, N-[(1S)-1-(Aminocarbonyl)-3-methylbutyl]-5-chloro-6-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]pyridine-3-carboxamide, 5-Chloro-N-(1-methylethyl)-6-[4-(3-oxo-2,3-dihydro-4H-1,4-benzoxazin-4-yl)piperidin-1-yl]pyridine-3-carboxamide, N-(1-Methylethyl)-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-[(1S)-1-(Aminocarbonyl)-2-methylbutyl]-3-nitro-4-[4-(2-oxo-2H-3,1-benzoxazin-1-yl)piperidin-1-yl]benzamide, N-(1-Methylethyl)-2-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-4-(trifluoromethyl)-5-pyrimidine-5-carboxamide, 3-Chloro-N-(1-methylethyl)-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, 3-Amino-N-(1-methylethyl)-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-(1-Methylethyl)-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-[(1S)-1-(Aminocarbonyl)-3-methylbutyl]-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, 3-(Ethylamino)-N-(1-methylethyl)-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, 3-(Diethylamino)-N-(1-methylethyl)-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, N-(1-Methylethyl)-3-[(methylsulfonyl)amnino]-4-[4-(2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]benzamide, 5-Chloro-N-(1-methylethyl)-6-[4-(2-oxo-1,3-benzoxazol-3(2H)-yl)piperidin-1-yl]pyridine-3-carboxamide, N-(1-Methylethyl)-3-nitro-4-[4-(2-oxo-3,4-dihydroquinolin-1(2H)-yl)piperidin-1-yl]benzamide, 4-[4-(7-Chloro-2-oxo-2H-3,1-benzoxazin-1(4H)-yl)piperidin-1-yl]-N-(1-methylethyl)-3-nitrobenzamide, 5-Chloro-N-(1-methylethyl)-6-[4-(2-oxo-3,4-dihydroquinolin-1(2H)-yl)piperidin-1-yl]pyridine-3-carboxamide, 4-[4-(2,2-Dioxido-3,4-dihydro-1H-2,1,3-benzothiadiazin-1-yl)piperidin-1-yl]-N-(1-methylethyl)-3-nitrobenzamide, or a pharmaceutically acceptable salt or solvate thereof.
 9. A pharmaceutical composition comprising a compound according to any one of claims 1 to 8 in combination with a pharmaceutically acceptable diluent, adjuvent or carrier.
 10. A compound according to any one of claims 1 to 8 for use in therapy.
 11. A compound according to any one of claims 1 to 8 for use in the treatment of rheumatoid arthritis.
 12. Use of a compound according to any one of claims 1 to 8 in the manufacture of a medicament for use in therapy.
 13. A method of effecting immunosuppression which comprises administering to a patient a therapeutically effective amount of a compound according to any one of claims 1 to
 8. 14. A process for the preparation of a compound of formula (I) which comprises reaction of a compound of formula (II):

where R, R¹, X, Y and Z are as defined in formula (I) or a protected derivative thereof, with a compound of formula (III):

where B and R² are as defined in formula (I) or a protected derivative thereof, and L is a leaving group, and optionally thereafter in any order: converting one or more functional groups into further functional groups removing any protecting groups forming a pharmaceutically acceptable salt or solvate. 